IRF4 Monoclonal antibody

IRF4 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1A12D5

Cat no : 66451-1-Ig

Synonyms

IRF 4, IRF4, LSIRF, Multiple myeloma oncogene 1, MUM1, NF EM5



Tested Applications

Positive WB detected inhuman spleen tissue, pig spleen tissue
Positive IHC detected inhuman tonsillitis
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC :
Sample-dependent, check data in validation data gallery

Product Information

66451-1-Ig targets IRF4 in WB, IHC,ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IRF4 fusion protein Ag25203
Full Name IRF 4
Calculated molecular weight 52 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC015752
Gene symbol IRF4
Gene ID (NCBI) 3662
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

Protocols

Product Specific Protocols
WB protocol for IRF4 antibody 66451-1-IgDownload protocol
IHC protocol for IRF4 antibody 66451-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols