IRF4 Monoclonal antibody

IRF4 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1A12D5

Cat no : 66451-1-Ig

Synonyms

IRF 4, IRF4, LSIRF, Multiple myeloma oncogene 1, MUM1, NF EM5

---

Validation Data Gallery

• 

  WB analysis of human spleen using 66451-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spleen using 66451-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66451-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66451-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: human spleen tissue, pig spleen tissue
• Positive IHC detected in: human tonsillitis; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

66451-1-Ig targets IRF4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IRF4 fusion protein Ag25203
• Full Name: IRF 4
• Calculated molecular weight: 52 kDa
• Observed molecular weight: 50 kDa
• GenBank accession number: BC015752
• Gene symbol: IRF4
• Gene ID (NCBI): 3662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

Publications

Species	Application	Title
human,mouse	WB,IF	Bioeng Transl Med; Macrophage-targeted delivery of siRNA to silence Mecp2 gene expression attenuates pulmonary fibrosis.; Authors - Yong Mou
human	WB,CoIP	Biochim Biophys Acta Mol Cell Res; RNF2 promotes the progression of colon cancer by regulating ubiquitination and degradation of IRF4; Authors - Jiwei Wang