IRF4 Monoclonal antibody

• Home
• Products
• Primary Antibodies
• IRF4 Monoclonal antibody (66451-1-Ig)

IRF4 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66451-1-Ig Copied!

Clone No. 1A12D5

Publications (10)

Download datasheet

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

MUM1/IRF4, 1A12D5, interferon regulatory factor 4, IRF 4, IRF-4

• 

  WB analysis of human spleen using 66451-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 66451-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 66451-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of pig spleen using 66451-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 66451-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 66451-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for IRF4 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: U266 cells, human spleen tissue, pig spleen tissue, rat spleen tissue, HuT 78 cells, MJ cells
• Positive IHC detected in: human tonsillitis; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66451-1-Ig targets IRF4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25203

  Product name: Recombinant human IRF4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-451 aa of BC015752

  Sequence: MNLEGGGRGGEFGMSAVSCGNGKLRQWLIDQIDSGKYPGLVWENEEKSIFRIPWKHAGKQDYNREEDAALFKAWALFKGKFREGIDKPDPPTWKTRLRCALNKSNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPPLDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGPACENGCQVTGTFYACAPPESQAPGVPTEPSIRSAEALAFSDCRLHICLYYREILVKELTTSSPEGCRISHGHTYDASNLDQVLFPYPEDNGQRKNIEKLLSHLERGVVLWMAPDGLYAKRLCQSRIYWDGPLALCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE

• Full Name: IRF 4
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 48-52 kDa
• GenBank Accession Number: BC015752
• Gene Symbol: IRF4
• Gene ID (NCBI): 3662
• ENSEMBL Gene ID: ENSG00000137265
• RRID: AB_2881820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15306
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

Publications

What published studies show

The anti-IRF4 antibody (Cat# 66451-1-Ig) has 10 total citations published in journals including Advanced Science, Oncogene, Free Radical Biology and Medicine, British Journal of Cancer, Inflammation Research, Bioengineering & Translational Medicine, Toxicology, Scientific Reports, BBA Molecular Cell Research, and FEBS Journal. Research fields span intestinal inflammation, myeloma progression, renal fibrosis, colorectal cancer, allergic asthma, pulmonary fibrosis, hematopoietic senescence, oncogene regulation, colon cancer, and macrophage polarization.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
pig	WB	Adv Sci (Weinh); Mechanisms of Baicalin Alleviates Intestinal Inflammation: Role of M1 Macrophage Polarization and Lactobacillus amylovorus; Authors - Shunfen Zhang
human	IF	Oncogene; LAMP5 modulates IRF4 stability and nuclear transport: a critical mechanism in myeloma progression and therapy.; Authors - Zou Li
rat	IHC	Free Radic Biol Med; Urban particulate matter induces NLRP3/NLRP6 inflammasomes, autophagy, senescence and fibrotic responses by increasing CSF2 signaling in kidney cells.; Authors - Ruei-Yu Su
mouse	WB	Br J Cancer; Impact of gut microbiome on radiotherapy and immunotherapy efficacy in microsatellite-stable colorectal cancer: role of propionic acid and B. fragilis; Authors - Lu Yu
		Inflamm Res; Ethyl ferulate attenuates allergic asthma via inhibiting macrophage M2 polarization through STAT6/IRF4 pathway.; Authors - Longlong Ge
mouse,human	WB,IF	Bioeng Transl Med; Macrophage-targeted delivery of siRNA to silence Mecp2 gene expression attenuates pulmonary fibrosis.; Authors - Yong Mou