IRF4 Monoclonal antibody

IRF4 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC, CoIP

Conjugate

Unconjugated

CloneNo.

1A12D5

Cat no : 66451-1-Ig

Synonyms

IRF 4, IRF4, LSIRF, Multiple myeloma oncogene 1, MUM1, NF EM5



Tested Applications

Positive WB detected inhuman spleen tissue, pig spleen tissue
Positive IHC detected inhuman tonsillitis
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC :
Sample-dependent, check data in validation data gallery

Product Information

66451-1-Ig targets IRF4 in WB, IHC, CoIP applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IRF4 fusion protein Ag25203
Full Name IRF 4
Calculated molecular weight 52 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC015752
Gene symbol IRF4
Gene ID (NCBI) 3662
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

Protocols

Product Specific Protocols
WB protocol for IRF4 antibody 66451-1-IgDownload protocol
IHC protocol for IRF4 antibody 66451-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Biochim Biophys Acta Mol Cell Res

RNF2 promotes the progression of colon cancer by regulating ubiquitination and degradation of IRF4

Authors - Jiwei Wang