IRF4 Monoclonal antibody, PBS Only

• 

  WB analysis of human spleen using 66451-1-Ig (same clone as 66451-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 66451-1-Ig (same clone as 66451-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

IRF4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66451-1-PBS

Clone No.1A12D5

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

MUM1/IRF4, 1A12D5, interferon regulatory factor 4, IRF 4, IRF-4

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Product Information

66451-1-PBS targets IRF4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25203

  Product name: Recombinant human IRF4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-451 aa of BC015752

  Sequence: MNLEGGGRGGEFGMSAVSCGNGKLRQWLIDQIDSGKYPGLVWENEEKSIFRIPWKHAGKQDYNREEDAALFKAWALFKGKFREGIDKPDPPTWKTRLRCALNKSNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPPLDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGPACENGCQVTGTFYACAPPESQAPGVPTEPSIRSAEALAFSDCRLHICLYYREILVKELTTSSPEGCRISHGHTYDASNLDQVLFPYPEDNGQRKNIEKLLSHLERGVVLWMAPDGLYAKRLCQSRIYWDGPLALCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE

• Full Name: IRF 4
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 48-52 kDa
• GenBank Accession Number: BC015752
• Gene Symbol: IRF4
• Gene ID (NCBI): 3662
• ENSEMBL Gene ID: ENSG00000137265
• RRID: AB_2881820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15306
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.