IRF5 Polyclonal antibody

IRF5 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10547-1-AP

Synonyms

interferon regulatory factor 5, IRF 5, IRF5, SLEB10



Tested Applications

Positive WB detected inJurkat cells, A549 cells, HeLa cells, human heart tissue, NIH/3T3 cells, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10547-1-AP targets IRF5 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity brovine, human, mouse, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF5 fusion protein Ag0841
Full Name interferon regulatory factor 5
Calculated molecular weight 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC004201
Gene symbol IRF5
Gene ID (NCBI) 3663
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). Alternative splice variant encoding different isoforms exist. Catalog#10547-1-AP is a rabbit polyclonal antibody raised against the C-terminal of human IRF5.

Protocols

Product Specific Protocols
WB protocol for IRF5 antibody 10547-1-APDownload protocol
IHC protocol for IRF5 antibody 10547-1-APDownload protocol
IF protocol for IRF5 antibody 10547-1-APDownload protocol
IP protocol for IRF5 antibody 10547-1-APDownload protocol
FC protocol for IRF5 antibody 10547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Am J Transplant

Characterization of Transfusion-Elicited Acute Antibody-Mediated Rejection in a Rat Model of Kidney Transplantation.

Authors - G Huang
humanIF

Br J Sports Med

Chronic inflammation is a feature of Achilles tendinopathy and rupture.

Authors - Stephanie Georgina Dakin
mouseWB,IF

Biochem Pharmacol

Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization.

Authors - Hong Lu
humanIHC

Acta Orthop

Rotator cuff repair with biological graft augmentation causes adverse tissue outcomes.

Authors - Mustafa S Rashid
brovineWB

J Vet Res

Echinacea Purpurea Extract (cichoric Acid) Exerts an Anti-inflammatory Effect on Yak PBMCs and Regulates the TLR4 Signalling Pathway.

Authors - Cai-Hua Xue
humanWB

Immunopharmacol Immunotoxicol

A study on the immunomodulation of polysaccharopeptide through the TLR4-TIRAP/MAL-MyD88 signaling pathway in PBMCs from breast cancer patients.

Authors - Wang Jing J