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  • KD/KO Validated

Integrin Beta 1 Polyclonal antibody

Integrin Beta 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12594-1-AP

Synonyms

CD29, FNRB, GPIIA, Integrin beta 1, ITB1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB



Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells
Positive IHC detected inhuman placenta tissue, mouse kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12594-1-AP targets Integrin Beta 1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, sika deer, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin Beta 1 fusion protein Ag3078
Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Calculated Molecular Weight 88 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC020057
Gene Symbol ITGB1
Gene ID (NCBI) 3688
RRIDAB_2130085
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Protocols

Product Specific Protocols
WB protocol for Integrin Beta 1 antibody 12594-1-APDownload protocol
IHC protocol for Integrin Beta 1 antibody 12594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Decellularized extracellular matrix scaffolds identify full-length collagen VI as a driver of breast cancer cell invasion in obesity and metastasis.

Authors - Andrew L Wishart
humanWB

Nat Commun

Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.

Authors - Yi-Ing Chen
humanImmunostaining

Sci Adv

Podocyte injury elicits loss and recovery of cellular forces.

Authors - Kathryn E Haley
humanWB

Adv Healthc Mater

Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein.

Authors - Moyuan Deng
  • KD Validated
humanWB

Sci Bull (Beijing)

The Rhinolophus affinis bat ACE2 and multiple animal orthologs are functional receptors for bat coronavirus RaTG13 and SARS-CoV-2.

Authors - Pei Li
humanWB

ACS Appl Mater Interfaces

A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells

Authors - Xinyang Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alberto (Verified Customer) (01-16-2024)

Multiple bands, very different compared to advertised picture. I want my money back.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: astrocyte cell line
Integrin Beta 1 Antibody Western Blot validation (1:500 dilution) in astrocyte cell line (Cat no:12594-1-AP)
FH

Udesh (Verified Customer) (07-10-2022)

The antibody worked great in Co-IP and WB. Great chunky band close to 130 kD. For Co-IP: 6uL/200ug protein and 1:2000 in 3%BSA for WB

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Bone Marrow MSCs
FH

Guorong (Verified Customer) (05-23-2022)

works better in ARPE-19 cells than 293T cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: 293T and ARPE-19
Integrin Beta 1 Antibody Western Blot validation (1:2000 dilution) in 293T and ARPE-19 (Cat no:12594-1-AP)
FH

Boyan (Verified Customer) (03-29-2019)

Good for WB - recognises a clean band around the expected molecular weight.

  • Applications: Western Blot,