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Integrin Beta 1 Polyclonal antibody
Integrin Beta 1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12594-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HT-1080 cells, HeLa cells |
Positive IHC detected in | human placenta tissue, human breast cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse liver tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 32 publications below |
IHC | See 8 publications below |
IF | See 14 publications below |
IP | See 1 publications below |
Product Information
12594-1-AP targets Integrin Beta 1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, bovine, chicken, sika deer |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Integrin Beta 1 fusion protein Ag3078 |
Full Name | integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) |
Calculated molecular weight | 88 kDa |
Observed molecular weight | 130 kDa |
GenBank accession number | BC020057 |
Gene symbol | ITGB1 |
Gene ID (NCBI) | 3688 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.
Protocols
Product Specific Protocols | |
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WB protocol for Integrin Beta 1 antibody 12594-1-AP | Download protocol |
IHC protocol for Integrin Beta 1 antibody 12594-1-AP | Download protocol |
IF protocol for Integrin Beta 1 antibody 12594-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Adv Decellularized extracellular matrix scaffolds identify full-length collagen VI as a driver of breast cancer cell invasion in obesity and metastasis. | ||
Nat Commun Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation. | ||
ACS Appl Mater Interfaces A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells | ||
Sci Bull (Beijing) The Rhinolophus affinis bat ACE2 and multiple animal orthologs are functional receptors for bat coronavirus RaTG13 and SARS-CoV-2. | ||
Theranostics Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Udesh (Verified Customer) (07-10-2022) | The antibody worked great in Co-IP and WB. Great chunky band close to 130 kD. For Co-IP: 6uL/200ug protein and 1:2000 in 3%BSA for WB
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FH Guorong (Verified Customer) (05-23-2022) | works better in ARPE-19 cells than 293T cells
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FH Boyan (Verified Customer) (03-29-2019) | Good for WB - recognises a clean band around the expected molecular weight.
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