Validation Data Gallery
|Positive WB detected in||HT-1080 cells, HeLa cells|
|Positive IHC detected in||human placenta tissue, mouse kidney tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12594-1-AP targets Integrin Beta 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, sika deer|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Integrin Beta 1 fusion protein Ag3078|
|Full Name||integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)|
|Calculated molecular weight||88 kDa|
|Observed molecular weight||130 kDa|
|GenBank accession number||BC020057|
|Gene ID (NCBI)||3688|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guorong (Verified Customer) (05-23-2022)
works better in ARPE-19 cells than 293T cells
Boyan (Verified Customer) (03-29-2019)
Good for WB - recognises a clean band around the expected molecular weight.