Integrin Beta 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Integrin Beta 1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

52

Reviews (3)

Cat no : 12594-1-AP

Synonyms

CD29, FNRB, GPIIA, Integrin beta 1, ITB1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB

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Validation Data Gallery

• 

  WB analysis using 12594-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 12594-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 12594-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12594-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12594-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 12594-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Integrin Beta 1 antibody (12594-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse liver using 12594-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Integrin Beta 1 antibody (12594-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HeLa cells
• Positive IHC detected in: human placenta tissue, human breast cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 32 publications below
• IHC: See 8 publications below
• IF: See 14 publications below
• IP: See 1 publications below

Product Information

12594-1-AP targets Integrin Beta 1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, chicken, sika deer
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin Beta 1 fusion protein Ag3078
• Full Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Calculated molecular weight: 88 kDa
• Observed molecular weight: 130 kDa
• GenBank accession number: BC020057
• Gene symbol: ITGB1
• Gene ID (NCBI): 3688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Publications

Species	Application	Title
human	WB	Sci Adv; Decellularized extracellular matrix scaffolds identify full-length collagen VI as a driver of breast cancer cell invasion in obesity and metastasis.; Authors - Andrew L Wishart
human	WB	Nat Commun; Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.; Authors - Yi-Ing Chen
human	Immunostaining	Sci Adv; Podocyte injury elicits loss and recovery of cellular forces.; Authors - Kathryn E Haley
human	WB	ACS Appl Mater Interfaces; A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells; Authors - Xinyang Zhao
human	WB	Sci Bull (Beijing); The Rhinolophus affinis bat ACE2 and multiple animal orthologs are functional receptors for bat coronavirus RaTG13 and SARS-CoV-2.; Authors - Pei Li
human	IF	Theranostics; Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.; Authors - Yinan Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (07-10-2022)

The antibody worked great in Co-IP and WB. Great chunky band close to 130 kD. For Co-IP: 6uL/200ug protein and 1:2000 in 3%BSA for WB

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Bone Marrow MSCs

FH

Guorong (Verified Customer) (05-23-2022)

works better in ARPE-19 cells than 293T cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T and ARPE-19

Integrin Beta 1 Antibody Western Blot validation (1:2000 dilution) in 293T and ARPE-19 (Cat no:12594-1-AP)

FH

Boyan (Verified Customer) (03-29-2019)

Good for WB - recognises a clean band around the expected molecular weight.

• Applications: Western Blot,

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