ITPRIP Polyclonal antibody

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  WB analysis of A549 using 26055-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26055-1-AP (ITPRIP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 26055-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26055-1-AP (ITPRIP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 26055-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26055-1-AP (ITPRIP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HuH-7 using 26055-1-AP

  IP result of anti-ITPRIP (IP:26055-1-AP, 4ug; Detection:26055-1-AP 1:800) with HuH-7 cells lysate 1200 ug.

• 

  IP experiment of A549 using 26055-1-AP

  IP result of anti-ITPRIP (IP:26055-1-AP, 4ug; Detection:26055-1-AP 1:800) with A549 cells lysate 640 ug.

ITPRIP Polyclonal Antibody for WB, IP, ELISA

Cat No. 26055-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

bA127L20, DANGER, Inositol 1,4,5-trisphosphate receptor-interacting protein, KIAA1754, Protein DANGER

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Tested Applications

• Positive WB detected in: A549 cells, MDA-MB-453s cells
• Positive IP detected in: A549 cells, HuH-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26055-1-AP targets ITPRIP in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22012

  Product name: Recombinant human ITPRIP protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 201-547 aa of BC070108

  Sequence: QVDRPLLCHLFVPFTPPEPYRFHPELWCSGRSVPLDRQGYGQIKVVRADGDTLSCICGKTKLGEDMLCLLHGRNSMAPPCGDMENLLCATDSLYLDTMQVMKWFQTALTRAWKGIAHKYEFDLAFGQLDSPGSLKIKFRSGKFMPFNLIPVIQCDDSDLYFVSHLPREPSEGTPASSTDWLLSFAVYERHFLRTTLKALPEGACHLSCLQIASFLLSKQSRLTGPSGLSSYHLKTALLHLLLLRQAADWKAGQLDARLHELLCFLEKSLLQKKLHHFFIGNRKVPEAMGLPEAVLRAEPLNLFRPFVLQRSLYRKTLDSFYEMLKNAPALISEYSLHVPSDQPTPKS

• Full Name: inositol 1,4,5-triphosphate receptor interacting protein
• Calculated Molecular Weight: 547 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC070108
• Gene Symbol: ITPRIP
• Gene ID (NCBI): 85450
• RRID: AB_2880354
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IWB1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITPRIP (inositol 1,4,5-trisphosphate receptor interacting protein) is a key regulatory protein that binds to IP3 receptors to modulate endoplasmic reticulum calcium release and intracellular calcium homeostasis. It is widely expressed in human tissues with high abundance in the central nervous system. ITPRIP participates in neurodevelopment, cell apoptosis, innate immunity and tumor progression. Its abnormal expression is closely linked to glioma malignancy, neurodevelopmental disorders and immune dysfunction, serving as an essential molecule in physiological regulation and disease pathogenesis. (PMID: 29899107)