Iduronate 2 sulfatase Monoclonal antibody, PBS Only

• 

  WB analysis of human placenta using 66112-1-Ig (same clone as 66112-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66112-1-Ig (same clone as 66112-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66112-1-Ig (same clone as 66112-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66112-1-Ig (same clone as 66112-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66112-1-Ig (same clone as 66112-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66112-1-Ig (same clone as 66112-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66112-1-Ig (same clone as 66112-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66112-1-Ig (same clone as 66112-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Iduronate 2 sulfatase antibody (66112-1-Ig, Clone: 1A3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

Iduronate 2 sulfatase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66112-1-PBS

Clone No.1A3F9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

IDS, 1A3F9, Alpha-L-iduronate sulfate sulfatase, EC:3.1.6.13, Iduronate 2-sulfatase

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Product Information

66112-1-PBS targets Iduronate 2 sulfatase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19095

  Product name: Recombinant human IDS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-312 aa of BC006170

  Sequence: MPPPRTGRGLLWLGLVLSSVCVALGSETQANSTTDALNVLLIIVDDLRPSLGCYGDKLVRSPNIDQLASHSLLFQNAFAQQAVCAPSRVSFLTGRRPDTTRLYDFNSYWRVHAGNFSTIPQYFKENGYVTMSVGKVFHPGISSNHTDDSPYSWSFPPYHPSSEKYENTKTCRGPDGELHANLLCPVDVLDVPEGTLPDKQSTEQAIQLLEKMKTSASPFFLAVGYHKPHIPFRYPKEFQKLYPLENITLAPDPEVPDGLPPVAYNPWMDIRQREDVQALNISVPYGPIPVDFQEDQSSTGFRLKTSSTRKYK

• Full Name: iduronate 2-sulfatase
• Calculated Molecular Weight: 312aa,35 kDa; 550aa,62 kDa
• Observed Molecular Weight: 76 kDa, 55 kDa
• GenBank Accession Number: BC006170
• Gene Symbol: IDS
• Gene ID (NCBI): 3423
• RRID: AB_2881511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P22304
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).