INS Monoclonal antibody

INS Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, Cell treatment, ELISA

Conjugate

Unconjugated

CloneNo.

4B6A7

Cat no : 66198-1-Ig

Synonyms

ILPR, INS, IRDN



Tested Applications

Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman pancreas tissue, mouse pancreas tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66198-1-Ig targets INS in WB, IHC, IF, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen INS fusion protein Ag8630
Full Name INS
Calculated molecular weight 110 aa, 12 kDa
GenBank accession numberBC005255
Gene symbol INS
Gene ID (NCBI) 3630
RRIDAB_2881591
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, pro-INS, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus. INS may also exist 36 kDa form corresponding to the hexameric INS form.

Protocols

Product Specific Protocols
IHC protocol for INS antibody 66198-1-IgDownload protocol
IF protocol for INS antibody 66198-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Front Immunol

Metabolic effects of CCL5 deficiency in lean and obese mice

Authors - Hui Zhou
mouseIF

Food Chem X

Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice.

Authors - Jingyu Fang
mouseIF

Clin Sci (Lond)

Mitochondrial protein MPV17 promotes β-cell apoptosis in diabetogenesis

Authors - Qiaoli Tang
mouseIF

FASEB J

cGAS-STING mediates cytoplasmic mitochondrial-DNA-induced inflammatory signal transduction during accelerated senescence of pancreatic β-cells induced by metabolic stress.

Authors - Huiqing Hu
mouseIF

Ecotoxicol Environ Saf

Green light exposure aggravates high-fat diet feeding-induced hepatic steatosis and pancreatic dysfunction in male mice.

Authors - Shiyao Zhang
mouseIF

J Cell Mol Med

SDF-1 inhibits the dedifferentiation of islet β cells in hyperglycaemia by up-regulating FoxO1 via binding to CXCR4.

Authors - Xiang-Yu Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Vincent (Verified Customer) (07-08-2019)

This antibody offers a good detection of Insulin expression in both DM mice and WT mice. The insulin expression can be observed in the islet cells of pancreatic tissue.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: pancreatic tissue/ islet cells
INS Antibody Immunofluorescence, validation (1:500 dilution) in pancreatic tissue/ islet cells (Cat no:66198-1-Ig)