Insulin Antibody

Insulin Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4B6A7

Cat no : 66198-1-Ig

Synonyms

ILPR, INS, Insulin, IRDN



Tested Applications

Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman pancreas tissue, mouse pancreas tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66198-1-Ig targets Insulin in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Insulin fusion protein Ag8630
Full Name insulin
Calculated molecular weight 110aa,12 kDa
Observed molecular weight
GenBank accession number BC005255
Gene symbol INS
Gene ID (NCBI) 3630
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in insulin results in type 1 diabetes mellitus. Insulin may also exist 36 kDa form corresponding to the hexameric insulin form.

Protocols

Product Specific Protocols
IHC protocol for Insulin antibody 66198-1-IgDownload protocol
IF protocol for Insulin antibody 66198-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Cell Death Dis

Glutathione prevents chronic oscillating glucose intake-induced β-cell dedifferentiation and failure.

Authors - Jitai Zhang
ratIHC

Diabetes Metab Syndr Obes

Chronic oscillating glucose challenges disarrange innate immune homeostasis to potentiate the variation of neutrophil-lymphocyte ratio in rats with or without hidden diabetes mellitus.

Authors - Gaoxiong Yang
ratIF

Biomol Ther (Seoul)

Involvement of Estrogen Receptor-α in the Activation of Nrf2-Antioxidative Signaling Pathways by Silibinin in Pancreatic β-Cells.

Authors - Chun Chu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Vincent (Verified Customer) (07-08-2019)

This antibody offers a good detection of Insulin expression in both DM mice and WT mice. The insulin expression can be observed in the islet cells of pancreatic tissue.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: pancreatic tissue/ islet cells
Insulin Antibody Immunofluorescence, validation (1:500 dilution) in pancreatic tissue/ islet cells (Cat no:66198-1-Ig)