CD103 Monoclonal antibody

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  IF Staining of human PBMCs using 68893-3-Ig

  Immunofluorescent analysis of un-fixed 5 µg/mL PHA ( 3 days) treated human PBMCs using Integrin alpha-E antibody (68893-3-Ig, Clone: 4B6G6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005).

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  FC experiment of human PBMCs using 68893-3-Ig

  1x10^6 untreated human PBMCs (left) and 5 µg/mL PHA (3 days) treated human PBMCs (right) were surface stained with 0.2 μg Integrin Alpha-E Monoclonal Antibody (68893-3-Ig, Clone: 4B6G6) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Cells were not fixed.

CD103 Monoclonal Antibody for IF/ICC, FC, ELISA

Cat No. 68893-3-Ig

Clone No.4B6G6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC, ELISA

Synonyms

4B6G6, HML 1 antigen, HML-1 antigen, HUMINAE, Integrin alpha E

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Tested Applications

• Positive IF/ICC detected in: 5 µg/mL PHA ( 3 days) treated human PBMCs
• Positive FC detected in: 5 µg/mL PHA for 3 days human PBMCs

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68893-3-Ig targets CD103 in IF/ICC, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27410

  Product name: Recombinant human Integrin alpha-E protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 147-456 aa of BC113436

  Sequence: LLDPDARVDTGDCYSNKEGGGEDDVNTARQRRALEKEEEEDKEEEEDEEEEEAGTEIAIILDGSGSIDPPDFQRAKDFISNMMRNFYEKCFECNFALVQYGGVIQTEFDLRDSQDVMASLARVQNITQVGSVTKTASAMQHVLDSIFTSSHGSRRKASKVMVVLTDGGIFEDPLNLTTVINSPKMQGVERFAIGVGEEFKSARTARELNLIASDPDETHAFKVTNYMALDGLLSKLRYNIISMEGTVGDALHYQLAQIGFSAQILDERQVLLGAVGAFDWSGGALLYDTRSRRGRFLNQTAAAAADAEAA

• Full Name: integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
• Calculated Molecular Weight: 1179 aa, 130 kDa
• GenBank Accession Number: BC113436
• Gene Symbol: ITGAE
• Gene ID (NCBI): 3682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P38570
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin alpha-E (CD103) is a member of the integrin family, specifically the αEβ7 heterodimer. It plays a crucial role in the immune system, particularly in the interaction between leukocytes and epithelial cells. Integrin alpha-E (CD103) is a critical component of the immune system, particularly in the context of mucosal immunity. Its ability to bind to E-cadherin and regulate the production of IFN-I in cDCs during viral infections highlights its multifaceted role in immune regulation and disease prevention. (PMID: 33584680)