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  • KD/KO Validated

Integrin Beta 5 Polyclonal antibody

Integrin Beta 5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28543-1-AP

Synonyms

FLJ26658, Integrin beta 5, integrin, beta 5, ITGB5



Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

28543-1-AP targets Integrin Beta 5 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin Beta 5 fusion protein Ag29783
Full Name integrin, beta 5
Calculated molecular weight 88 kDa
Observed molecular weight100 kDa
GenBank accession numberBC006541
Gene symbol ITGB5
Gene ID (NCBI) 3693
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Integrin Beta 5 antibody 28543-1-APDownload protocol
IHC protocol for Integrin Beta 5 antibody 28543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Aging Cell

Fibronectin type III domain-containing 5 improves aging-related cardiac dysfunction in mice.

Authors - Can Hu
  • KD Validated
humanWB,IHC

Pharmacol Res

Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex

Authors - Li Gu
  • KD Validated
ratIF

Exp Brain Res

MFGE8 mitigates brain injury in a rat model of SAH by maintaining vascular endothelial integrity via TIGβ5/PI3K/CXCL12 signaling.

Authors - Jikai Wang
mouseWB

Technol Cancer Res Treat

In Vivo Visualized Tracking of Tumor-Derived Extracellular Vesicles Using CRISPR-Cas9 System.

Authors - Yangyang Ye