JPH4 Polyclonal antibody

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  WB analysis using 32471-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32471-1-AP (JPH4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

JPH4 Polyclonal Antibody for WB, ELISA

Cat No. 32471-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KIAA1831, Junctophilin-like 1 protein, Junctophilin-4, JPHL1, JP-4

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32471-1-AP targets JPH4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38425

  Product name: Recombinant human JPH4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 450-600 aa of NM_001146028.1

  Sequence: GLTPSEGSPELPSSPASSRQPWRPPACRSPLPPGGDQGPFSSPKAWPEEWGGAGAQAEELAGYEAEDEAGMQGPGPRDGSPLLGGCSDSSGSLREEEGEDEEPLPPLRAPAGTEPEPIAMLVLRGSSSRGPDAGCLTEELGEPAATERPAQ

• Full Name: junctophilin 4
• Calculated Molecular Weight: 66kDa，628aa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_001146028.1
• Gene Symbol: JPH4
• Gene ID (NCBI): 84502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96JJ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

JPH4 (Junctophilin 4) is a member of the junctophilin family of transmembrane proteins. JPH4 is involved in the formation of junctional membrane complexes between the plasma membrane and the endoplasmic/sarcoplasmic reticulum in excitable cells. It plays a crucial role in maintaining the structural integrity and function of these complexes. JPH4 is biased in expression in the brain (RPKM 34.3) and endometrium (RPKM 8.7), among other tissues.