ATG13 Monoclonal antibody

ATG13 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


Human, Mouse, Rat, Pig and More (1)







Cat no : 66708-1-Ig


ATG13, Autophagy related protein 13, FLJ20698, KIAA0652

Tested Applications

Positive WB detected inHEK-293 cells, BGC-823 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells, Jurkat cells
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66708-1-Ig targets ATG13 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATG13 fusion protein Ag12968
Full Name KIAA0652
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC001331
Gene Symbol KIAA0652
Gene ID (NCBI) 9776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)


Product Specific Protocols
WB protocol for ATG13 antibody 66708-1-IgDownload protocol
IHC protocol for ATG13 antibody 66708-1-IgDownload protocol
IF protocol for ATG13 antibody 66708-1-IgDownload protocol
FC protocol for ATG13 antibody 66708-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Rep

Destabilization of TP53 by USP10 is essential for neonatal autophagy and survival

Authors - Hongchang Li