KIAA1033 Polyclonal antibody

KIAA1033 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 51101-1-AP

Synonyms

WASHC4, WASH complex subunit SWIP, WASH complex subunit 7, WASH complex subunit 4, SWIP

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Validation Data Gallery

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  WB analysis using 51101-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51101-1-AP (KIAA1033 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 51101-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51101-1-AP (KIAA1033 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 51101-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 51101-1-AP (KIAA1033 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 51101-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 51101-1-AP (KIAA1033 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

51101-1-AP targets KIAA1033 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: KIAA1033
• Calculated Molecular Weight: 136.4 kDa
• Observed Molecular Weight: 136 kDa
• GenBank Accession Number: BC104994
• Gene Symbol: KIAA1033
• Gene ID (NCBI): 23325
• RRID: AB_2881248
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA1033, also named as WASH-interacting protein and SWIP, is a component of WASH complex. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. KIAA1033 has two isoforms with MW 100 kDa and 136 kDa.

Publications

Species	Application	Title
mouse	WB	Pigment Cell Melanoma Res; Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour.; Authors - Benjamin J Tyrrell
human	WB	Autophagy; RAB21 controls autophagy and cellular energy homeostasis by regulating retromer-mediated recycling of SLC2A1/GLUT1; Authors - Yifei Pei