Tested Applications
| Positive WB detected in | A549 cells, HeLa cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
51101-1-AP targets KIAA1033 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | KIAA1033 |
| Calculated Molecular Weight | 136.4 kDa |
| Observed Molecular Weight | 136 kDa |
| GenBank Accession Number | BC104994 |
| Gene Symbol | KIAA1033 |
| Gene ID (NCBI) | 23325 |
| RRID | AB_2881248 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2M389 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KIAA1033, also named as WASH-interacting protein and SWIP, is a component of WASH complex. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. KIAA1033 has two isoforms with MW 100 kDa and 136 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KIAA1033 antibody 51101-1-AP | Download protocol |
| WB protocol for KIAA1033 antibody 51101-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KIAA1033 (WASH-interacting protein/SWIP) polyclonal antibody (Cat# 51101-1-AP) has 7 citations across Autophagy, MedComm, Mol Cell Proteomics, Immunology, Sci Rep, and Pigment Cell Melanoma Res. Research fields include autophagy, endosomal trafficking, WNT signaling, esophageal tumorigenesis, metabolic rewiring/mTORC1, antigen internalization, astrocyte dysfunction, and WASH complex function in melanocytic lineage.
| Species | Application | Title |
|---|---|---|
Autophagy WNT2B impairs endosomal trafficking via WASHC5 to inhibit autophagy: a novel non-secretory WNT pathway. | ||
Autophagy RAB21 controls autophagy and cellular energy homeostasis by regulating retromer-mediated recycling of SLC2A1/GLUT1 | ||
MedComm (2020) FBXW7 Targets the SPT6-ΔNp63 Axis for Degradation to Inhibit Esophageal Tumorigenesis Induced by 4-Nitroquinoline N-Oxide. | ||
Mol Cell Proteomics Metabolic Enzyme Alterations and Astrocyte Dysfunction in a Murine Model of Alexander Disease With Severe Reactive Gliosis. | ||
Immunology SNX17 Regulates Antigen Internalisation and Phagosomal Maturation by Dendritic Cells | ||
Reviews
What customers say
The antibody performed well for Western Blotting. Users reported a band at the expected size with good intensity, using a 1:500 dilution on HeLaM cells. Overall, the product was rated favorably for its reliable WB performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ioana (Verified Customer) (08-28-2025) | It worked well for Western Blotting, band size as expected and good intensity.
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