KIF15 antibody (55407-1-PBS)

Filter: AllWBIPIHCFC (Intra)

WB analysis of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)
WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HEK-293 cells. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 55407-1-AP (same clone as 55407-1-PBS)
WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected Jurkat cells. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.
WB analysis using 55407-1-AP (same clone as 55407-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.
WB analysis of mouse thymus using 55407-1-AP (same clone as 55407-1-PBS)
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.
WB analysis of MDA-MB-453s using 55407-1-AP (same clone as 55407-1-PBS)
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.
IP experiment of mouse thymus using 55407-1-AP (same clone as 55407-1-PBS)
IP result of anti-KIF15 (IP:55407-1-AP, 4ug; Detection:55407-1-AP 1:500) with mouse thymus tissue lysate 2800ug. This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 55407-1-AP (same clone as 55407-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 55407-1-AP (same clone as 55407-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.
FC experiment of HEK-293 using 55407-1-AP (same clone as 55407-1-PBS)
1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug KIF15 Polyclonal antibody (55407-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.
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1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug KIF15 Polyclonal antibody (55407-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 55407-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

55407-1-PBS targets KIF15 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	kinesin family member 15
Calculated Molecular Weight	160 kDa
Observed Molecular Weight	150-165 kDa
GenBank Accession Number	NM_020242
Gene Symbol	KIF15
Gene ID (NCBI)	56992
RRID	AB_11182836
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NS87
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)