KIF15 Polyclonal antibody

• Featured Product
• KD/KO Validated

KIF15 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 55407-1-AP

Synonyms

KNSL7, KLP2, Kinesin-like protein KIF15, Kinesin-like protein 7, Kinesin-like protein 2

---

Validation Data Gallery

• 

  WB analysis using 55407-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55407-1-AP

  WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HEK-293 cells.

• 

  WB analysis of Jurkat using 55407-1-AP

  WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected Jurkat cells.

• 

  WB analysis of mouse thymus using 55407-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 55407-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse thymus using 55407-1-AP

  IP result of anti-KIF15 (IP:55407-1-AP, 4ug; Detection:55407-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

• 

  IHC staining of human testis using 55407-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 55407-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 55407-1-AP

  Immunofluorescent analysis of HEK-293 cells using 55407-1-AP (KIF15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 55407-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug KIF15 Polyclonal antibody (55407-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: Jurkat cells, mouse thymus tissue, HEK-293 cells, MDA-MB-453s cells
• Positive IP detected in: mouse thymus tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 22 publications below
• IHC: See 13 publications below
• IF: See 6 publications below

Product Information

55407-1-AP targets KIF15 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: kinesin family member 15
• Calculated Molecular Weight: 160 kDa
• Observed Molecular Weight: 150-165 kDa
• GenBank Accession Number: NM_020242
• Gene Symbol: KIF15
• Gene ID (NCBI): 56992
• RRID: AB_11182836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)

Publications

Species	Application	Title
human	WB	Am J Respir Crit Care Med; Rare and Common Variants in KIF15 Contribute to Genetic Risk of Idiopathic Pulmonary Fibrosis; Authors - David Zhang
human	IHC,WB	Cancer Res; KIF15-mediated stabilization of AR and AR-V7 contributes to enzalutamide resistance in prostate cancer.; Authors - Lin Gao ; KD Validated
human	IHC	Cancer Cell Int; Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer; Authors - Enkui Zhang
human	WB,IHC	Cell Physiol Biochem; Increased KIF15 Expression Predicts a Poor Prognosis in Patients with Lung Adenocarcinoma.; Authors - Yuan Qiao ; KD Validated
mouse	WB	Front Cell Dev Biol; Transcriptional, Post-Transcriptional, and Post-Translational Mechanisms Rewrite the Tubulin Code During Cardiac Hypertrophy and Failure.; Authors - Sai Aung Phyo
human	WB,IHC	Front Oncol; KIF15 Promotes Progression of Castration Resistant Prostate Cancer by Activating EGFR Signaling Pathway.; Authors - Lin Gao ; KD Validated