Validation Data Gallery
|Positive WB detected in||mouse thymus tissue, Jurkat cells|
|Positive IP detected in||mouse thymus tissue|
|Positive IHC detected in||human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
55407-1-AP targets KIF15 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||kinesin family member 15|
|Calculated molecular weight||160 kDa|
|Observed molecular weight||150-165 kDa|
|GenBank accession number||NM_020242|
|Gene ID (NCBI)||56992|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)
|Product Specific Protocols|
|WB protocol for KIF15 antibody 55407-1-AP||Download protocol|
|IHC protocol for KIF15 antibody 55407-1-AP||Download protocol|
|IF protocol for KIF15 antibody 55407-1-AP||Download protocol|
|IP protocol for KIF15 antibody 55407-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Involvement of C-terminal truncation mutation of kinesin-5 in resistance to kinesin-5 inhibitor.
Exp Eye Res
KIF15 plays a role in promoting the tumorigenicity of melanoma.
Cancer Manag Res
KIF15 promotes bladder cancer proliferation via the MEK-ERK signaling pathway.
KIF15 contributes to cell proliferation and migration in breast cancer.
KIF11 and KIF15 mitotic kinesins are potential therapeutic vulnerabilities for malignant peripheral nerve sheath tumors.
Cell Death Dis
B7-H3 regulates KIF15-activated ERK1/2 pathway and contributes to radioresistance in colorectal cancer.