Validation Data Gallery
|Positive WB detected in||Jurkat cells, mouse thymus tissue, HEK-293 cells, MDA-MB-453s cells|
|Positive IP detected in||mouse thymus tissue|
|Positive IHC detected in||human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
55407-1-AP targets KIF15 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Full Name||kinesin family member 15|
|Calculated molecular weight||160 kDa|
|Observed molecular weight||150-165 kDa|
|GenBank accession number||NM_020242|
|Gene ID (NCBI)||56992|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)
|Product Specific Protocols|
|WB protocol for KIF15 antibody 55407-1-AP||Download protocol|
|IHC protocol for KIF15 antibody 55407-1-AP||Download protocol|
|IF protocol for KIF15 antibody 55407-1-AP||Download protocol|
|IP protocol for KIF15 antibody 55407-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Am J Respir Crit Care Med
Rare and Common Variants in KIF15 Contribute to Genetic Risk of Idiopathic Pulmonary Fibrosis
KIF15-mediated stabilization of AR and AR-V7 contributes to enzalutamide resistance in prostate cancer.
Cancer Cell Int
Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer
Cell Physiol Biochem
Increased KIF15 Expression Predicts a Poor Prognosis in Patients with Lung Adenocarcinoma.
Front Cell Dev Biol
Transcriptional, Post-Transcriptional, and Post-Translational Mechanisms Rewrite the Tubulin Code During Cardiac Hypertrophy and Failure.
KIF15 Promotes Progression of Castration Resistant Prostate Cancer by Activating EGFR Signaling Pathway.