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  • KD/KO Validated

KIF15 Polyclonal antibody

KIF15 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 55407-1-AP

Synonyms

HKLP2, KIF15, kinesin family member 15, Kinesin like protein 2, Kinesin like protein 7, Kinesin like protein KIF15, KLP2, KNSL7, NY BR 62



Tested Applications

Positive WB detected inmouse thymus tissue, Jurkat cells
Positive IP detected inmouse thymus tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55407-1-AP targets KIF15 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name kinesin family member 15
Calculated molecular weight 160 kDa
Observed molecular weight 150-165 kDa
GenBank accession numberNM_020242
Gene symbol KIF15
Gene ID (NCBI) 56992
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)

Protocols

Product Specific Protocols
WB protocol for KIF15 antibody 55407-1-APDownload protocol
IHC protocol for KIF15 antibody 55407-1-APDownload protocol
IF protocol for KIF15 antibody 55407-1-APDownload protocol
IP protocol for KIF15 antibody 55407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Involvement of C-terminal truncation mutation of kinesin-5 in resistance to kinesin-5 inhibitor.

Authors - Eri Saeki
humanWB,IHC,IF

Exp Eye Res

KIF15 plays a role in promoting the tumorigenicity of melanoma.

Authors - Xiaoyu Yu
  • KD Validated
humanWB,IHC

Cancer Manag Res

KIF15 promotes bladder cancer proliferation via the MEK-ERK signaling pathway.

Authors - Hongda Zhao
  • KD Validated
humanWB,IHC

Hum Cell

KIF15 contributes to cell proliferation and migration in breast cancer.

Authors - Xiaokang Gao
humanWB,IHC

Neurooncol Adv

KIF11 and KIF15 mitotic kinesins are potential therapeutic vulnerabilities for malignant peripheral nerve sheath tumors.

Authors - Ernest Terribas
humanIHC,WB,IF

Cell Death Dis

B7-H3 regulates KIF15-activated ERK1/2 pathway and contributes to radioresistance in colorectal cancer.

Authors - Yanchao Ma
  • KD Validated