WB analysis using 13105-1-AP (same clone as 13105-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13105-1-AP (KIF2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 13105-1-AP (KIF2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 13105-1-AP (same clone as 13105-1-PBS)
WB result of KIF2A antibody (13105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF2A transfected HeLa cells. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
WB result of KIF2A antibody (13105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF2A transfected HeLa cells. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 13105-1-AP (same clone as 13105-1-PBS)
IP result of anti-KIF2A (IP:13105-1-AP, 4ug; Detection:13105-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
IP result of anti-KIF2A (IP:13105-1-AP, 4ug; Detection:13105-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 13105-1-AP (same clone as 13105-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A549 cells using KIF2A antibody (13105-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed A549 cells using KIF2A antibody (13105-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
IF Staining of MDCK using 13105-1-AP (same clone as 13105-1-PBS)
Immunofluorescent analysis of MDCK cells using 13105-1-AP (KIF2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of MDCK cells using 13105-1-AP (KIF2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
FC experiment of A549 using 13105-1-AP (same clone as 13105-1-PBS)
1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KIF2A (13105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KIF2A (13105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
WB analysis using 13105-1-AP (same clone as 13105-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13105-1-AP (KIF2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 13105-1-AP (same clone as 13105-1-PBS)
WB result of KIF2A antibody (13105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF2A transfected HeLa cells. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
IHC Figures
IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
IP Figures
IP experiment of mouse brain using 13105-1-AP (same clone as 13105-1-PBS)
IP result of anti-KIF2A (IP:13105-1-AP, 4ug; Detection:13105-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of A549 using 13105-1-AP (same clone as 13105-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A549 cells using KIF2A antibody (13105-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
IF Staining of MDCK using 13105-1-AP (same clone as 13105-1-PBS)
Immunofluorescent analysis of MDCK cells using 13105-1-AP (KIF2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
FC (INTRA) Figures
FC experiment of A549 using 13105-1-AP (same clone as 13105-1-PBS)
1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KIF2A (13105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
KIF2A Polyclonal antibody
Catalog Number
13105-1-PBS
Citations
-
Dilutions
Applications
WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Reactivity
human, mouse, rat, canine
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
