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  • KD/KO Validated

KIF7 Polyclonal antibody

KIF7 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 24693-1-AP

Synonyms

KIF7, kinesin family member 7, Kinesin like protein KIF7, UNQ340



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24693-1-AP targets KIF7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF7 fusion protein Ag19119
Full Name kinesin family member 7
Calculated Molecular Weight 1343 aa, 151 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC112271
Gene Symbol KIF7
Gene ID (NCBI) 374654
RRIDAB_2879677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF7(Kinesin family member 7) is a microtubule interacting protein that functions to regulate Hh signaling by maintaining the architecture of the primary cilium, a complex microtubule-based organelle with many signal transduction functions (PMID: 26439735). KIF7 has been shown to have both negative and positive roles in Shh signal transduction. Without a ligand, KIF7 localizes to the cilium base where it forms a complex with Gli proteins and other pathway components and promotes the processing of GliRs (PMID: 21552264).

Protocols

Product Specific Protocols
WB protocol for KIF7 antibody 24693-1-APDownload protocol
IP protocol for KIF7 antibody 24693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Adv

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Authors - Chuan Chen
humanWB

Cells

Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.

Authors - Damian Kołat
humanWB

Thorac Cancer

Human papillomavirus 16 (HPV 16) E6 but not E7 inhibits the antitumor activity of LKB1 in lung cancer cells by downregulating the expression of KIF7.

Authors - Yue Hu
  • KD Validated
humanWB,IHC

Cell Death Dis

Circular RNA circUBE2J2 acts as the sponge of microRNA-370-5P to suppress hepatocellular carcinoma progression.

Authors - Lu Zhang
humanWB

Proteome Sci

Proteomics identifies differentially expressed proteins in glioblastoma U87 cells treated with hederagenin

Authors - Yesen Zhang
mouse,humanWB,IF

Leukemia

P4HA2 hydroxylates SUFU to regulate the paracrine Hedgehog signaling and promote B-cell lymphoma progression

Authors - Quanfu Li