Filter: AllWBIPIHCIF/ICC

WB analysis using 21017-1-AP (same clone as 21017-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
WB analysis using 21017-1-AP (same clone as 21017-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
WB analysis using 21017-1-AP (same clone as 21017-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
WB analysis using 21017-1-AP (same clone as 21017-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
WB analysis of human testis using 21017-1-AP (same clone as 21017-1-PBS)
human testis tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 21017-1-AP (same clone as 21017-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
WB analysis of HT-1080 using 21017-1-AP (same clone as 21017-1-PBS)
HT-1080 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 21017-1-AP (same clone as 21017-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
WB analysis of mouse colon using 21017-1-AP (same clone as 21017-1-PBS)
mouse colon tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
WB analysis of mouse small intestine using 21017-1-AP (same clone as 21017-1-PBS)
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 21017-1-AP (same clone as 21017-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 21017-1-AP (KLF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 21017-1-AP (same clone as 21017-1-PBS)
IP result of anti-KLF5 (IP:21017-1-AP, 4ug; Detection:21017-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
IHC staining of mouse embryo using 21017-1-AP (same clone as 21017-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21017-1-AP (KLF5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 21017-1-AP (same clone as 21017-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 21017-1-AP (same clone as 21017-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21017-1-AP (KLF5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
IHC staining of human bowen disease using 21017-1-AP (same clone as 21017-1-PBS)
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21017-1-AP (KLF5 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
IHC staining of human bowen disease using 21017-1-AP (same clone as 21017-1-PBS)
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21017-1-AP (KLF5 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
IHC staining of mouse embryo using 21017-1-AP (same clone as 21017-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21017-1-AP (KLF5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 21017-1-AP (same clone as 21017-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KLF5 antibody (21017-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 21017-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

KLF5 Polyclonal antibody, PBS Only

KLF5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 21017-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

21017-1-PBS targets KLF5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15267 Product name: Recombinant human KLF5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC042131 Sequence: MATRVLSMSARLGPVPQPPAPQDEPVFAQLKPVLGAANPARDAALFPGEELKHAHHRPQAQPAPAQAPQPAQPPATGPRLPPEDLVQTRCEMEKYLTPQLPPVPIIPEHKKYRRDSASVVDQFFTDTEGLPYSINMNVFLPDITHLRTGLYKSQRPCVTHIKTEPVAIFSHQSETTAPPPAPTQALPEFTSIFSSHQTAAPEVNNIFIKQELPTPDLHLSVPTQQGHLYQLLNTPDLDMPSSTNQTAAMDTLNVSMSAAMAGLNTHTSAVPQTAVKQFQGMPPCTYTMPSQFLPQQATYFPPSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPTTLPVN Predict reactive species
Full Name	Kruppel-like factor 5 (intestinal)
Calculated Molecular Weight	457 aa, 51 kDa
Observed Molecular Weight	51-55 kDa
GenBank Accession Number	BC042131
Gene Symbol	KLF5
Gene ID (NCBI)	688
RRID	AB_10696447
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13887
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).