KLHL14 Polyclonal antibody

KLHL14 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16693-1-AP

Synonyms

Protein interactor of Torsin-1A, Printor, Protein interactor of torsinA, KIAA1384, Kelch-like protein 14

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Validation Data Gallery

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  WB analysis of PC-3 using 16693-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16693-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16693-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16693-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16693-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16693-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 16693-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16693-1-AP (KLHL14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, HepG2 cells, MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16693-1-AP targets KLHL14 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLHL14 fusion protein Ag10065
• Full Name: kelch-like 14 (Drosophila)
• Calculated Molecular Weight: 44 kDa, 71 kDa
• Observed Molecular Weight: 35 kDa, 70 kDa
• GenBank Accession Number: BC021267
• Gene Symbol: KLHL14
• Gene ID (NCBI): 57565
• RRID: AB_2131169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kelch-like protein 14 (KLHL14) is a member of the KLHL family. The kelch motif was initially discovered in Kelch. In this protein there are six copies of the motif. It has been shown for one member that it is related to Galactose Oxidase for which a structure has been solved. Two isoforms of this protein exist - 70kDa and 43kDa.