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KLHL3 Polyclonal antibody

KLHL3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 16951-1-AP


kelch like 3 (Drosophila), Kelch like protein 3, KIAA1129, KLHL3

Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, Jurkat cells, rat liver tissue, human liver tissue, mouse thymus tissue, HL-60 cells, mouse pancreas tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16951-1-AP targets KLHL3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL3 fusion protein Ag10580
Full Name kelch-like 3 (Drosophila)
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC034035
Gene Symbol KLHL3
Gene ID (NCBI) 26249
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for KLHL3 antibody 16951-1-APDownload protocol
IHC protocol for KLHL3 antibody 16951-1-APDownload protocol
IP protocol for KLHL3 antibody 16951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Genet

KLHL3 mutations cause familial hyperkalemic hypertension by impairing ion transport in the distal nephron.

Authors - Louis-Dit-Picard Hélène H

Cell Death Differ

A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.

Authors - Xiaoyu Qi

J Am Soc Nephrol

Combined Kelch-Like 3 and Cullin 3 Degradation is a Central Mechanism in Familial Hyperkalemic Hypertension in Mice.

Authors - Yujiro Maeoka

J Am Soc Nephrol

Kelch-Like Protein 2 Mediates Angiotensin II-With No Lysine 3 Signaling in the Regulation of Vascular Tonus.

Authors - Moko Zeniya

J Am Soc Nephrol

Renal COP9 Signalosome Deficiency Alters CUL3-KLHL3-WNK Signaling Pathway.

Authors - Ryan J Cornelius

Hum Mol Genet

Impaired degradation of WNK1 and WNK4 kinases causes PHAII in mutant KLHL3 knock-in mice.

Authors - Koichiro Susa