• Featured Product
  • KD/KO Validated

KLHL3 Polyclonal antibody

KLHL3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16951-1-AP

Synonyms

kelch like 3 (Drosophila), Kelch like protein 3, KIAA1129, KLHL3



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, Jurkat cells, rat liver tissue, human liver tissue, mouse thymus tissue, HL-60 cells, mouse pancreas tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16951-1-AP targets KLHL3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL3 fusion protein Ag10580
Full Name kelch-like 3 (Drosophila)
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC034035
Gene symbol KLHL3
Gene ID (NCBI) 26249
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KLHL3 antibody 16951-1-APDownload protocol
IHC protocol for KLHL3 antibody 16951-1-APDownload protocol
IP protocol for KLHL3 antibody 16951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Biochem J

p62/SQSTM1-mediated selective autophagy is involved in KLHL3-dependent WNK4 degradation.

Authors - Yutaro Mori
humanWB

Biochem Biophys Res Commun

Impaired degradation of WNK by Akt and PKA phosphorylation of KLHL3.

Authors - Yuki Yoshizaki
mouseWB

Clin Exp Nephrol

Decreased KLHL3 expression is involved in the pathogenesis of pseudohypoaldosteronism type II caused by cullin 3 mutation in vivo.

Authors - Sayaka Yoshida
  • KO Validated
humanWB,IF

Exp Cell Res

cMyBP-C was decreased via KLHL3-mediated proteasomal degradation in congenital heart diseases.

Authors - Leitong Wang
  • KD Validated
mouseWB

Mol Cell Biol

KLHL3 knockout mice reveal the physiological role of KLHL3 and the pathophysiology of PHAII caused by mutant KLHL3.

Authors - Emi Sasaki
  • KO Validated
mouseIF

Hum Mol Genet

Impaired degradation of WNK1 and WNK4 kinases causes PHAII in mutant KLHL3 knock-in mice.

Authors - Koichiro Susa