|Positive WB detected in||HepG2 cells, mouse liver tissue, Jurkat cells, rat liver tissue, human liver tissue, mouse thymus tissue, HL-60 cells, mouse pancreas tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human liver tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16951-1-AP targets KLHL3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLHL3 fusion protein Ag10580|
|Full Name||kelch-like 3 (Drosophila)|
|Calculated molecular weight||65 kDa|
|Observed molecular weight||65 kDa|
|GenBank accession number||BC034035|
|Gene ID (NCBI)||26249|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
p62/SQSTM1-mediated selective autophagy is involved in KLHL3-dependent WNK4 degradation.
Biochem Biophys Res Commun
Impaired degradation of WNK by Akt and PKA phosphorylation of KLHL3.
Clin Exp Nephrol
Decreased KLHL3 expression is involved in the pathogenesis of pseudohypoaldosteronism type II caused by cullin 3 mutation in vivo.
Exp Cell Res
cMyBP-C was decreased via KLHL3-mediated proteasomal degradation in congenital heart diseases.
Mol Cell Biol
KLHL3 knockout mice reveal the physiological role of KLHL3 and the pathophysiology of PHAII caused by mutant KLHL3.
Hum Mol Genet
Impaired degradation of WNK1 and WNK4 kinases causes PHAII in mutant KLHL3 knock-in mice.