KLK11 Recombinant monoclonal antibody

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  WB analysis of TT using 86517-1-RR

  TT cells were subjected to SDS PAGE followed by western blot with 86517-1-RR (KLK11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity of 86517-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86517-1-RR against Human KLK11 were performed. The affinity constant is below 1 pM.

KLK11 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86517-1-RR

Clone No.251323F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Kallikrein 11, EC:3.4.21.-, Kallikrein11, Kallikrein-11, Kallikrein-11 inactive chain 1

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Tested Applications

• Positive WB detected in: TT cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86517-1-RR targets KLK11 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2890

  Product name: Recombinant Human KLK11 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 19-250 aa of NM_144947.2

  Sequence: AAKEPGARSSPLQAMRILQLILLALATGLVGGETRIIKGFECKPHSQPWQAALFEKTRLLCGATLIAPRWLLTAAHCLKPRYIVHLGQHNLQKEEGCEQTRTATESFPHPGFNNSLPNKDHRNDIMLVKMASPVSITWAVRPLTLSSRCVTAGTSCLISGWGSTSSPQLRLPHTLRCANITIIEHQKCENAYPGNITDTMVCASVQEGGKDSCQGDSGGPLVCNQSLQGIIS

• Full Name: kallikrein-related peptidase 11
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: NM_144947.2
• Gene Symbol: KLK11
• Gene ID (NCBI): 11012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UBX7-2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.