KLK15 Polyclonal antibody

KLK15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27515-1-AP

Synonyms

ACO, ACO protease, HSRNASPH, Kallikrein 15, KLK15

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Validation Data Gallery

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  WB analysis of SMMC-7721 using 27515-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 27515-1-AP (KLK15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 27515-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27515-1-AP (KLK15 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 27515-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27515-1-AP (KLK15 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 27515-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27515-1-AP (KLK15 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 27515-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27515-1-AP (KLK15 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: SMMC-7721 cells
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27515-1-AP targets KLK15 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLK15 fusion protein Ag26232
• Full Name: kallikrein-related peptidase 15
• Calculated Molecular Weight: 255 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC069480
• Gene Symbol: KLK15
• Gene ID (NCBI): 55554
• RRID: AB_2880895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.