KLK3/PSA Polyclonal antibody

KLK3/PSA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10679-1-AP

Synonyms

APS, Gamma seminoprotein, hK3, Kallikrein 3, kallikrein related peptidase 3, KLK2A1, KLK3, KLK3/PSA, P 30 antigen, Prostate specific antigen, PSA, Semenogelase, Seminin



Tested Applications

Positive WB detected inLNCaP cells, Transfected HEK-293 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

10679-1-AP targets KLK3/PSA in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLK3/PSA fusion protein Ag1060
Full Name kallikrein-related peptidase 3
Calculated molecular weight 29 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC005307
Gene symbol KLK3
Gene ID (NCBI) 354
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for KLK3/PSA antibody 10679-1-APDownload protocol
IHC protocol for KLK3/PSA antibody 10679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Theranostics

ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3.

Authors - Lijie Zhou
mouseIHC

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou
humanWB

Elife

A feedback loop between the androgen receptor and 6-phosphogluoconate dehydrogenase (6PGD) drives prostate cancer growth

Authors - Joanna L Gillis
humanWB

Mol Cancer Ther

PMID: 28802253

Authors - Linh N K Tran
mouseWB,IF

Stem Cell Res Ther

Expansion of mouse castration-resistant intermediate prostate stem cells in vitro

Authors - Yalan Xu
humanWB

Cancer Metab

Prostate cancer cell proliferation is influenced by LDL-cholesterol availability and cholesteryl ester turnover.

Authors - Nikki L Raftopulos