KLK3/PSA Polyclonal antibody

KLK3/PSA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 10679-1-AP


APS, Gamma seminoprotein, hK3, Kallikrein 3, kallikrein related peptidase 3, KLK2A1, KLK3, KLK3/PSA, P 30 antigen, Prostate specific antigen, PSA, Semenogelase, Seminin

Tested Applications

Positive WB detected inLNCaP cells, Transfected HEK-293 cells
Positive IP detected inLNCaP cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inLNCaP cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10679-1-AP targets KLK3/PSA in WB,IP,IHC,IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLK3/PSA fusion protein Ag1060
Full Name kallikrein-related peptidase 3
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC005307
Gene Symbol KLK3
Gene ID (NCBI) 354
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms  produced by alternative splicing.


Product Specific Protocols
WB protocol for KLK3/PSA antibody 10679-1-APDownload protocol
IHC protocol for KLK3/PSA antibody 10679-1-APDownload protocol
IF protocol for KLK3/PSA antibody 10679-1-APDownload protocol
IP protocol for KLK3/PSA antibody 10679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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