KLRG1 Polyclonal antibody

KLRG1 Polyclonal Antibody for WB, ELISA

Cat No. 10974-1-AP

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Synonyms

MAFA 2F1, MAFA, Killer cell lectin-like receptor subfamily G member 1, ITIM-containing receptor MAFA-L, C-type lectin domain family 15 member A

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

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WB analysis using 10974-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human peripheral blood leukocyte using 10974-1-AP
Human peripheral blood leukocytes were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Raji using 10974-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 10974-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10974-1-AP (KLRG1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 10974-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10974-1-AP (KLRG1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (3)

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MSDS SDS

Tested Applications

Positive WB detected in

Jurkat cells, Raji cells, human peripheral blood leukocyte, NK-92 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10974-1-AP targets KLRG1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1407 Product name: Recombinant human KLRG1 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-195 aa of BC012621 Sequence: MTDSVIYSMLELPTATQAQNDYGPQQKSSSSRPSCSCLVAIALGLLTAVLLSVLLYQWILCQGSNYSTCASCPSCPDRWMKYGNHCYYFSVEEKDWNSSLEFCLARDSHLLVITDNQEMSLLQVFLSEAFCWIGLRNNSGWRWEDGSPLNFSRISSNSFVQTCGAINKNGLQASSCEVPLHWVCKKCPFADQALF Predict reactive species
Full Name	killer cell lectin-like receptor subfamily G, member 1
Calculated Molecular Weight	22 kDa
Observed Molecular Weight	60-70 kDa
GenBank Accession Number	BC012621
Gene Symbol	KLRG1
Gene ID (NCBI)	10219
RRID	AB_2234383
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96E93
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLRG1 (killer cell lectin-like receptor subfamily G, member 1) is a C-type lectin inhibitory receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. KLRG1 is expressed by subsets of NK and T cells, existing both as a monomer and as a disulfide-linked homodimer (PMID: 10741410; 19604491). It is considered to be a cell differentiation marker for NK and T cells and is strongly induced by viral and other infections (PMID: 20373518). Through interactions with members of the cadherin family, KLRG1 plays an inhibitory role in NK- and T-cell function (PMID: 19009530; 20373518). Because of the glycosylation and disulfide bonds of the KLRG1 proteins, the observed molecular weights are typically 30-38 kDa (PMID: 17404256).

Publications

What published studies show

The KLRG1 Polyclonal antibody (Cat# 10974-1-AP) has 3 total citations, appearing in Brain (2 papers) and Journal of Neurology (1 paper). The cited studies focus on inclusion body myositis, examining mitochondrial damage and early immune responses, highly differentiated cytotoxic T cells, and morphological/molecular comparisons between HIV-associated and sporadic inclusion body myositis. The associated research fields span neuromuscular disorders and immunology.

Species	Application	Title
human		Brain Mitochondrial damage is associated with an early immune response in inclusion body myositis. Authors - D Felix Kleefeld View Article
human		Brain Highly differentiated cytotoxic T cells in inclusion body myositis. Authors - Steven A Greenberg View Article
human	IF	J Neurol Morphological and molecular comparison of HIV-associated and sporadic inclusion body myositis Authors - Sinja Vogt View Article