KMT2C Polyclonal antibody

KMT2C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 28437-1-AP

Synonyms

MLL3, Lysine N methyltransferase 2C, KIAA1506, Homologous to ALR protein, HALR

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Validation Data Gallery

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  WB analysis using 28437-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 28437-1-AP (KMT2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 28437-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28437-1-AP (KMT2C antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 28437-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28437-1-AP (KMT2C antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

28437-1-AP targets KMT2C in WB, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KMT2C fusion protein Ag28875
• Full Name: myeloid/lymphoid or mixed-lineage leukemia 3
• Calculated Molecular Weight: 541 kDa
• Observed Molecular Weight: 541kDa, 269 kDa
• GenBank Accession Number: NM_170606
• Gene Symbol: KMT2C
• Gene ID (NCBI): 58508
• RRID: AB_2918165
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lysine (K)-specific methyltransferase 2C (KMT2C, also known as MLL3) belongs to the mixed-lineage leukemia (MLL) family of histone methyltransferases which methylate the histone 3 tail at lysine 4 (H3K4) as part of the complex proteins associated with Set 1 (COMPASS) complex. Although originally identified as oncogenic fusions in leukemia, genome-wide mutation studies have revealed frequent, presumably loss-of-function, mutations in various members of the MLL family, including MLL2/KMT2B, MLL3/KMT2C, and MLL4/KMT2D in a variety of malignancies, particularly solid tumors. Mechanistic studies of KMT2C in normal cells have focused primarily on its role in enhancer regulation by deposition of H3K4me1 marks.

Publications

Species	Application	Title
human	WB	Int Immunopharmacol; Polysaccharides from Platycodonis Radix ameliorated respiratory syncytial virus-induced epithelial cell apoptosis and inflammation through activation of miR-181a-mediated Hippo and SIRT1 pathways.; Authors - Juan-Juan Li
human	ChIP	J Biol Chem; TRIM28 represses renal cell carcinoma cell proliferation by inhibiting TFE3/KDM6A-regulated autophagy; Authors - Tanjing Song
human	WB,IF,CoIP	Adv Sci (Weinh); TAOK3 Facilitates Esophageal Squamous Cell Carcinoma Progression and Cisplatin Resistance Through Augmenting Autophagy Mediated by IRGM; Authors - Mingchuang Sun
human	WB	Nat Chem Biol; UFL1 triggers replication fork degradation by MRE11 in BRCA1/2-deficient cells; Authors - Tian Tian