KMT2D Polyclonal antibody

• Featured Product
• KD/KO Validated

KMT2D Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 27266-1-AP

Synonyms

MLL2, KMT2D/MLL2

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Validation Data Gallery

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  WB analysis using 27266-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27266-1-AP (KMT2D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27266-1-AP (KMT2D antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 27266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27266-1-AP (KMT2D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human lymphoma tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• ChIP: See 3 publications below

Product Information

27266-1-AP targets KMT2D in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KMT2D fusion protein Ag26195
• Full Name: myeloid/lymphoid or mixed-lineage leukemia 2
• Calculated Molecular Weight: 593 kDa
• Observed Molecular Weight: 593 kDa
• GenBank Accession Number: NM_003482
• Gene Symbol: KMT2D/MLL2
• Gene ID (NCBI): 8085
• RRID: AB_2880823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KMT2D (a COMPASS/ Set1 family member; also known as MLL4, ALR, and MLL2) is the biggest H3K4 methyltransferase among the most frequently mutated genes in many different types of cancer (PMID: 34194626). The enzymatic function of KMT2D depends on a cluster of C-terminal conserved domains, including a PHD domain, two FY-rich motifs (FYRC and FYRN) and a catalytic SET domain. KMT2D has two frequently occurring genomic alterations: truncation and missense mutations. Loss of KMT2D is a bona fide tumor suppressor gene in FL and DLBCL. Kmt2d perturbed the expression of genes that sustain proliferation and survival, and the KMT2D protein directly binds and associates with an active chromatin conformation in negative modulators of the BCR and lymphocyte migration pathways, which in turn could affect B cell responses to antigen(PMID: 26366712).

Publications

Species	Application	Title
human	WB	Cancer Immunol Res; Molecular Subgroups of Intrahepatic Cholangiocarcinoma Discovered by Single-Cell RNA Sequencing-Assisted Multiomics Analysis.; Authors - Xuanwen Bao
human	IHC	Oncogene; Histone 3 lysine-27 demethylase KDM6A coordinates with KMT2B to play an oncogenic role in NSCLC by regulating H3K4me3.; Authors - Xuejiao Leng
human	IHC,IF	Cell Biosci; Histone methyltransferase KMT2D cooperates with MEF2A to promote the stem-like properties of oral squamous cell carcinoma.; Authors - Xinmiao Wang
human	IHC	Thorac Cancer; RNF213 gene mutation in circulating tumor DNA detected by targeted next-generation sequencing in the assisted discrimination of early-stage lung cancer from pulmonary nodules.; Authors - Ning Jiang
human	WB,IHC	Transl Cancer Res; Histone methyltransferase KMT2D mediated lipid metabolism via peroxisome proliferator-activated receptor gamma in prostate cancer; Authors - Qiliang Zhai
	ChIP	Cell Death Discov; Pseudogene ACTBP2 increases blood-brain barrier permeability by promoting KHDRBS2 transcription through recruitment of KMT2D/WDR5 in Aβ1-42 microenvironment.; Authors - Qianshuo Liu