Kininogen 1 Polyclonal antibody

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  WB analysis of HeLa using 11926-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11926-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 11926-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11926-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 11926-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11926-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11926-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 11926-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11926-1-AP (Kininogen 1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 11926-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11926-1-AP (Kininogen 1 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human endometrial cancer using 11926-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 11926-1-AP (Kininogen 1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human endometrial cancer using 11926-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 11926-1-AP (Kininogen 1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 11926-1-AP

  Immunofluorescent analysis of HepG2 cells, using KNG1 antibody 11926-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Kininogen 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11926-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

KNG1, Ile Ser Bradykinin Bradykinin, HMWK, Fitzgerald factor, Bradykinin

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Tested Applications

• Positive WB detected in: HeLa cells, A2780 cells, COLO 320 cells, MCF-7 cells, mouse kidney tissue
• Positive IHC detected in: human kidney tissue, human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

11926-1-AP targets Kininogen 1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kininogen 1 fusion protein Ag2574
• Full Name: kininogen 1
• Calculated Molecular Weight: 644 aa, 72 kDa
• Observed Molecular Weight: 48 kDa, 72 kDa
• GenBank Accession Number: BC060039
• Gene Symbol: Kininogen 1
• Gene ID (NCBI): 3827
• RRID: AB_2133403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01042
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This KNG1 gene contains 10 exons and encodes two proteins through alternative splicing: high molecular weight kininogen (HMWK) (72kDa) and low molecular weight kininogen (LMWK) (48kDa) (PMID: 37442062). HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein. 11926-1-AP was generated against N-terminal 300 aa of HMW kininogen. It can bind both HMW and LMW kiniogen.

Publications

Species	Application	Title
human	WB,CoIP	EBioMedicine; An HBV susceptibility variant of KNG1 modulates the therapeutic effects of interferons α and λ1 in HBV infection by promoting MAVS lysosomal degradation; Authors - Bin Zhang
human	WB	Cell Physiol Biochem; Novel Serum Biomarkers Detected by Protein Array in Polycystic Ovary Syndrome with Low Progesterone Level.; Authors - Qin Zheng
rat	WB	PLoS One; Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system.; Authors - Bo Ma
rat	WB	Biol Pharm Bull; Proteomic analysis reveals the renoprotective effect of Tribulus terrestris against obesity-related glomerulopathy in rats.; Authors - Yue-Hua Jiang
mouse	WB	Med Sci Monit; Isobaric Tagging for Relative and Absolute Protein Quantification (iTRAQ)-Based Quantitative Proteomics Analysis of Differentially Expressed Proteins 1 Week After Spinal Cord Injury in a Rat Model.; Authors - Shen Liu
rat	WB,IF	JID Innov; Kininogen-Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation.; Authors - Ting Wang