KRAS Polyclonal antibody, PBS Only

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  WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 12063-1-AP (same clone as 12063-1-PBS)

  WB result of KRAS antibody (12063-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KRAS transfected HEK-293 cells. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 12063-1-AP (same clone as 12063-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 12063-1-AP (same clone as 12063-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 12063-1-AP (same clone as 12063-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 12063-1-AP (same clone as 12063-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 12063-1-AP (same clone as 12063-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat kidney using 12063-1-AP (same clone as 12063-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IP experiment of MKN-45 using 12063-1-AP (same clone as 12063-1-PBS)

  IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:8000) with MKN-45 cells lysate 1560 ug. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HGC-27 using 12063-1-AP (same clone as 12063-1-PBS)

  IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:8000) with HGC-27 cells lysate 1520 ug. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

  IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:20000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

  IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:20000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12063-1-AP (same clone as 12063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12063-1-AP (same clone as 12063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 12063-1-AP (same clone as 12063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12063-1-AP (same clone as 12063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12063-1-AP (same clone as 12063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRAS antibody (12063-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KRAS (12063-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

KRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12063-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

pan RAS, C K RAS, c Ki ras, c-Ki-ras, c-K-ras

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Product Information

12063-1-PBS targets KRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2700

  Product name: Recombinant human KRAS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-188 aa of BC013572

  Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM

• Full Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Calculated Molecular Weight: 188 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC013572
• Gene Symbol: KRAS
• Gene ID (NCBI): 3845
• RRID: AB_878040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01116
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.