Cytokeratin 10 Polyclonal antibody

Cytokeratin 10 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 18343-1-AP

Synonyms

CK 10, CK10, Cytokeratin 10, K10, keratin 10, KPP, KRT10



Tested Applications

Positive IHC detected inhuman breast cancer tissue, human skin cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18343-1-AP is Cytokeratin 10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 10 fusion protein Ag13136
Full Name keratin 10
Calculated molecular weight 584 aa, 59 kDa
GenBank accession numberBC034697
Gene symbol KRT10
Gene ID (NCBI) 3858
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.

Protocols

Product Specific Protocols
IHC protocol for Cytokeratin 10 antibody 18343-1-APDownload protocol
FC protocol for Cytokeratin 10 antibody 18343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Lett

Cytokeratin expression in epidermal stem cells in skin adnexal tumors.

Authors - Xiaoqiu Zhou
mouseIHC

Life Sci

The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model.

Authors - Shidi Wu
mouseIF

Aging Cell

Nociceptive transient receptor potential canonical 7 (TRPC7) mediates aging-associated tumorigenesis induced by ultraviolet B.

Authors - Wen-Li Hsu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Bright staining with minimal background.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Keratinocytes
Cytokeratin 10 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:18343-1-AP)