Cytokeratin 10 Polyclonal antibody
Cytokeratin 10 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 18343-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse skin tissue, A431 cells, rat skin tissue |
Positive IHC detected in | human cervical cancer tissue, mouse skin tissue, rat skin tissue, human lung cancer tissue, human breast cancer tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
Positive FC (Intra) detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IHC | See 3 publications below |
IF | See 10 publications below |
Product Information
18343-1-AP targets Cytokeratin 10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Cytokeratin 10 fusion protein Ag13136 |
Full Name | keratin 10 |
Calculated Molecular Weight | 584 aa, 59 kDa |
Observed Molecular Weight | 50-59 kDa |
GenBank Accession Number | BC034697 |
Gene Symbol | Cytokeratin 10 |
Gene ID (NCBI) | 3858 |
RRID | AB_10863654 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Cytokeratin 10 antibody 18343-1-AP | Download protocol |
IHC protocol for Cytokeratin 10 antibody 18343-1-AP | Download protocol |
IF protocol for Cytokeratin 10 antibody 18343-1-AP | Download protocol |
FC protocol for Cytokeratin 10 antibody 18343-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Mol Biol Lett The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis | ||
Aging Cell Nociceptive transient receptor potential canonical 7 (TRPC7) mediates aging-associated tumorigenesis induced by ultraviolet B. | ||
Life Sci The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model. | ||
mBio Commensal-Related Changes in the Epidermal Barrier Function Lead to Alterations in the Benzo[a]Pyrene Metabolite Profile and Its Distribution in 3D Skin. | ||
Eur Surg Res A novel combination of keratinocyte and autologous microskin grafting to repair full thickness skin loss | ||
Int J Biol Macromol Skin-adaptive film dressing with smart-release of growth factors accelerated diabetic wound healing |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Joshua (Verified Customer) (07-27-2019) | Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Bright staining with minimal background.
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