Cytokeratin 10 Polyclonal antibody

Cytokeratin 10 Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 18343-1-AP


Cell and organelle markers, CK 10, CK10, Cytokeratin 10, Cytoskeleton Marker, K10, keratin 10, KPP, KRT10

Tested Applications

Positive WB detected inmouse skin tissue, A431 cells, rat skin tissue
Positive IHC detected inhuman cervical cancer tissue, mouse skin tissue, rat skin tissue, human lung cancer tissue, human breast cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18343-1-AP targets Cytokeratin 10 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 10 fusion protein Ag13136
Full Name keratin 10
Calculated Molecular Weight 584 aa, 59 kDa
Observed Molecular Weight50-59 kDa
GenBank Accession NumberBC034697
Gene Symbol KRT10
Gene ID (NCBI) 3858
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.


Product Specific Protocols
WB protocol for Cytokeratin 10 antibody 18343-1-APDownload protocol
IHC protocol for Cytokeratin 10 antibody 18343-1-APDownload protocol
FC protocol for Cytokeratin 10 antibody 18343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Mol Biol Lett

The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis

Authors - Tao Yang

Aging Cell

Nociceptive transient receptor potential canonical 7 (TRPC7) mediates aging-associated tumorigenesis induced by ultraviolet B.

Authors - Wen-Li Hsu

Life Sci

The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model.

Authors - Shidi Wu


Commensal-Related Changes in the Epidermal Barrier Function Lead to Alterations in the Benzo[a]Pyrene Metabolite Profile and Its Distribution in 3D Skin.

Authors - Lisa Lemoine

Eur Surg Res

A novel combination of keratinocyte and autologous microskin grafting to repair full thickness skin loss

Authors - Kai Yin

Int J Biol Macromol

Skin-adaptive film dressing with smart-release of growth factors accelerated diabetic wound healing

Authors - Yingzheng Zhao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Bright staining with minimal background.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Keratinocytes
Cytokeratin 10 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:18343-1-AP)