|Positive WB detected in||A431 cells, mouse skin tissue|
|Positive IHC detected in||human lung cancer tissue, human skin tissue, human cervical cancer tissue, human breast hyperplasia tissue, human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells|
|Positive FC detected in||A431 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:400-1:800|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
60320-1-Ig targets Cytokeratin 14 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, pig, rat samples.
|Tested Reactivity||human, mouse, pig, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytokeratin 14 fusion protein Ag17559|
|Full Name||keratin 14|
|Calculated molecular weight||472 aa, 52 kDa|
|Observed molecular weight||52 kDa|
|GenBank accession number||BC002690|
|Gene ID (NCBI)||3861|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.
|Product Specific Protocols|
|WB protocol for Cytokeratin 14 antibody 60320-1-Ig||Download protocol|
|IHC protocol for Cytokeratin 14 antibody 60320-1-Ig||Download protocol|
|IF protocol for Cytokeratin 14 antibody 60320-1-Ig||Download protocol|
|FC protocol for Cytokeratin 14 antibody 60320-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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