Cytokeratin 14 Monoclonal antibody

Cytokeratin 14 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

2G1E2

Cat no : 60320-1-Ig

Synonyms

CK 14, CK14, Cytokeratin 14, EBS3, EBS4, K14, keratin 14, KRT14, NFJ



Tested Applications

Positive WB detected inA431 cells, mouse skin tissue
Positive IHC detected inhuman lung cancer tissue, human skin tissue, human cervical cancer tissue, human breast hyperplasia tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:800
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

60320-1-Ig targets Cytokeratin 14 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 14 fusion protein Ag17559
Full Name keratin 14
Calculated molecular weight 472 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC002690
Gene symbol KRT14
Gene ID (NCBI) 3861
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 14 antibody 60320-1-IgDownload protocol
IHC protocol for Cytokeratin 14 antibody 60320-1-IgDownload protocol
IF protocol for Cytokeratin 14 antibody 60320-1-IgDownload protocol
FC protocol for Cytokeratin 14 antibody 60320-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Breast Cancer Res

Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation.

Authors - Dongdong Wang
mouseWB,IF

iScience

Cysteine Dioxygenase Regulates the Epithelial Morphogenesis of Mammary Gland via Cysteine Sulfinic Acid.

Authors - Jianjun Zhao
mouseIF

Stem Cell Reports

SOX2 Is a Marker for Stem-like Tumor Cells in Bladder Cancer.

Authors - Fengyu Zhu
mouseWB,IHC

Environ Sci Technol

Graphene Quantum Dots Disrupt Embryonic Stem Cell Differentiation by Interfering with the Methylation Level of Sox2.

Authors - Tingting Ku
mouseIF

Small

Eco-Friendly and Scalable Synthesis of Fullerenols with High Free Radical Scavenging Ability for Skin Radioprotection.

Authors - Maoru Zhao