Cytokeratin 14 Monoclonal antibody
Cytokeratin 14 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig, rat
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2G1E2
Cat no : 60320-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, mouse skin tissue |
Positive IHC detected in | human lung cancer tissue, human cervical cancer tissue, human skin tissue, human breast hyperplasia tissue, human skin cancer tissue, rat skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A431 cells |
Positive FC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:800 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 4 publications below |
IHC | See 2 publications below |
IF | See 9 publications below |
Product Information
60320-1-Ig targets Cytokeratin 14 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
Tested Reactivity | human, mouse, pig, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Cytokeratin 14 fusion protein Ag17559 |
Full Name | keratin 14 |
Calculated molecular weight | 472 aa, 52 kDa |
Observed molecular weight | 52 kDa |
GenBank accession number | BC002690 |
Gene symbol | KRT14 |
Gene ID (NCBI) | 3861 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Cytokeratin 14 antibody 60320-1-Ig | Download protocol |
IHC protocol for Cytokeratin 14 antibody 60320-1-Ig | Download protocol |
IF protocol for Cytokeratin 14 antibody 60320-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Small Eco-Friendly and Scalable Synthesis of Fullerenols with High Free Radical Scavenging Ability for Skin Radioprotection. | ||
Environ Sci Technol Graphene Quantum Dots Disrupt Embryonic Stem Cell Differentiation by Interfering with the Methylation Level of Sox2. | ||
J Nanobiotechnology Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration. | ||
Stem Cell Res Ther Bone marrow mesenchymal stem cells facilitate diabetic wound healing through the restoration of epidermal cell autophagy via the HIF-1α/TGF-β1/SMAD pathway | ||
Cell Biosci Single cell transcriptomic analysis of human amnion identifies cell-specific signatures associated with membrane rupture and parturition. |