CoraLite® Plus 647-conjugated Cytokeratin 14 Monoclonal antibody

Cytokeratin 14 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2G1E2

Cat no : CL647-60320

Synonyms

CK 14, CK14, Cytokeratin 14, EBS3, EBS4, K14, keratin 14, KRT14, NFJ

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Validation Data Gallery

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  FC experiment of A431 using CL647-60320

  1X10^6 A431 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Cytokeratin 14 (CL647-60320, Clone:2G1E2) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: A431 cells

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Product Information

CL647-60320 targets Cytokeratin 14 in FC (Intra) applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 14 fusion protein Ag17559
• Full Name: keratin 14
• Calculated molecular weight: 472 aa, 52 kDa
• Observed molecular weight: 52 kDa
• GenBank accession number: BC002690
• Gene symbol: KRT14
• Gene ID (NCBI): 3861
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.