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WB analysis of Jurkat using 18131-1-AP (same clone as 18131-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.
WB analysis using 18131-1-AP (same clone as 18131-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 18131-1-AP (Kindlin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.
WB analysis using 18131-1-AP (same clone as 18131-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 18131-1-AP (Kindlin 3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 18131-1-AP (same clone as 18131-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.
WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.
WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.
WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.
WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 18131-1-AP (same clone as 18131-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 18131-1-AP (same clone as 18131-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.
IF Staining of THP-1 using 18131-1-AP (same clone as 18131-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Kindlin 3 antibody (18131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.
IF Staining of Raji using 18131-1-AP (same clone as 18131-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed Raji cells using Kindlin 3 antibody (18131-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

Kindlin 3 Polyclonal antibody, PBS Only

Kindlin 3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 18131-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FERMT3, KIND3, Kindlin-3, MIG 2, MIG2 like protein

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

18131-1-PBS targets Kindlin 3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12713 Product name: Recombinant human FERMT3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 321-663 aa of BC004347 Sequence: DDLDVALSNLEVKLEGSAPTDVLDSLTTIPELKDHLRIFRPRKLTLKGYRQHWVVFKETTLSYYKSQDEAPGDPIQQLNLKGCEVVPDVNVSGQKFCIKLLVPSPEGMSEIYLRCQDEQQYARWMAGCRLASKGRTMADSSYTSEVQAILAFLSLQRTGSGGPGNHPHGPDASAEGLNPYGLVAPRFQRKFKAKQLTPRILEAHQNVAQLSLAEAQLRFIQAWQSLPDFGISYVMVRFKGSRKDEILGIANNRLIRIDLAVGDVVKTWRFSNMRQWNVNWDIRQVAIEFDEHINVAFSCVSASCRIVHEYIGGYIFLSTRERARGEELDEDLFLQLTGGHEAF Predict reactive species
Full Name	fermitin family homolog 3 (Drosophila)
Calculated Molecular Weight	76 kDa
Observed Molecular Weight	72-76 kDa
GenBank Accession Number	BC004347
Gene Symbol	Kindlin 3/FERMT3
Gene ID (NCBI)	83706
RRID	AB_2103056
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86UX7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Kindlin-3 (FERMT-3) is known to be central in hemostasis and thrombosis control and its deficiency disrupts platelet aggregation and causes Leukocyte Adhesion Deficiency disease. Point mutations in the kindlin-3 gene have been identified in humans with a rare inherited Integrin Activation efficiency Disease (IADD), also designated as Leukocyte Adhesion Deficiency syndrome (LAD-III or LADI variant). Kindlin-3 expression is restricted to cells of hematopoietic origin.