Kir6.2 Polyclonal antibody, PBS Only

• 

  WB analysis of rat heart using 16920-1-AP (same clone as 16920-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (Kir6.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16920-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 16920-1-AP (same clone as 16920-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (Kir6.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16920-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16920-1-AP (same clone as 16920-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16920-1-AP (KCNJ11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16920-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 16920-1-AP (same clone as 16920-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (KCNJ11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16920-1-PBS in a different storage buffer formulation.

Kir6.2 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 16920-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

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Product Information

16920-1-PBS targets Kir6.2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10262

  Product name: Recombinant human Kir6.2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 169-390 aa of BC064497

  Sequence: MKTAQAHRRAETLIFSKHAVIALRHGRLCFMLRVGDLRKSMIISATIHMQVVRKTTSPEGEVVPLHQVDIPMENGVGGNSIFLVAPLIIYHVIDANSPLYDLAPSDLHHHQDLEIIVILEGVVETTGITTQARTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTIKVPTPLCTARQLDEDHSLLEALTLASARGPLRKRSVPMAKAKPKFSISPDSLS

• Full Name: potassium inwardly-rectifying channel, subfamily J, member 11
• Calculated Molecular Weight: 390 aa, 44 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC064497
• Gene Symbol: Kir6.2
• Gene ID (NCBI): 3767
• RRID: AB_2130461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14654
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Kir6.2 (also known as BIR or IKATP), encoded by the KCNJ11 gene, is the pore-forming unit of the ATP-sensitive K+ channel, an inward-rectifier potassium ion channel. Kir6.2 is controlled by G-proteins and is found associated with the sulfonylurea receptor (SUR) to constitute the ATP-sensitive K+ channel. The KCNJ11 gene is located at 11p15.1 and has no intron. Mutations in KCNJ11 are a cause of familial PHHI, an autosomal recessive disorder characterized by unregulated ins secretion. Defects in KCNJ11 may also contribute to autosomal dominant non-ins-dependent diabetes mellitus type II (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM).