L1CAM Polyclonal antibody

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  WB analysis using 32885-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32885-1-AP (L1CAM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 32885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 32885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 32885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 32885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

L1CAM Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32885-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

L1cam, N-CAM-L1, CAML1, CD171, N CAM L1

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
• Positive IHC detected in: mouse cerebellum tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32885-1-AP targets L1CAM in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: L1CAM fusion protein Eg3022
• Full Name: L1 cell adhesion molecule
• Calculated Molecular Weight: 141 kDa
• Observed Molecular Weight: 200-220 kDa, 140 kDa
• GenBank Accession Number: N/A
• Gene Symbol: L1cam
• Gene ID (NCBI): 16728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P11627
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503). It has been reported that L1CAM can be proteolytically cleaved into a soluble fragment of 140 kDa and an intracellular fragment of 80-85 kDa (PMID: 30842511; 34380733).