LAG-3/CD223 Polyclonal antibody

LAG-3/CD223 Polyclonal Antibody for IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 16616-1-AP

Synonyms

LAG-3, CD223, lymphocyte activation gene 3, LAG3, LAG 3



Tested Applications

Positive IP detected inmouse liver tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16616-1-AP targets LAG-3/CD223 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAG-3/CD223 fusion protein Ag9909
Full Name lymphocyte-activation gene 3
Calculated Molecular Weight 525 aa, 57 kDa
Observed Molecular Weight 54 kDa, 60-65 kDa
GenBank Accession NumberBC052589
Gene Symbol LAG-3
Gene ID (NCBI) 3902
RRIDAB_2133350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

Protocols

Product Specific Protocols
IHC protocol for LAG-3/CD223 antibody 16616-1-APDownload protocol
IF protocol for LAG-3/CD223 antibody 16616-1-APDownload protocol
IP protocol for LAG-3/CD223 antibody 16616-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Immunol Immunother

Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.

Authors - Pei Yang
humanIHC

Ann Transl Med

Single-cell RNA-seq reveals transcriptional landscape and intratumor heterogenicity in gallbladder cancer liver metastasis microenvironment.

Authors - Yigang Zhang
humanIHC

Front Oncol

The immune checkpoint expression in the tumor immune microenvironment of DLBCL: Clinicopathologic features and prognosis

Authors - Jiajia Ma
humanWB

Int J Oncol

Effect of DPP4/CD26 expression on SARS‑CoV‑2 susceptibility, immune response, adenosine (derivatives m62A and CD) regulations on patients with cancer and healthy individuals

Authors - Jiaman Du
humanWB

Br J Ophthalmol

Implications of LAG3 and CTLA4 immune checkpoints beyond PD-1/PD-L1 as a potential target in determining the prognosis of uveal melanoma patients

Authors - Seema Kashyap
humanWB

J Clin Invest

Targeting fibrinogen-like protein 1 enhances immunotherapy in hepatocellular carcinoma

Authors - Mingen Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Eman (Verified Customer) (09-20-2021)

the antibody works very well for WB the recommended dilution is 1:500 to 1:1000 i think we can even dilute it more this antibody is higly recommended

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 overnight
  • Cell Tissue Type: HEK293T cells
LAG-3/CD223 Antibody Western Blot validation (1:1000 overnight dilution) in HEK293T cells (Cat no:16616-1-AP)