LAG-3/CD223 Polyclonal antibody, PBS Only

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  IP experiment of mouse liver using 16616-1-AP (same clone as 16616-1-PBS)

  IP result of anti-LAG-3 (IP:16616-1-AP, 4ug; Detection:16616-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16616-1-AP (LAG-3/CD223 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16616-1-AP (LAG-3/CD223 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAG-3 antibody (16616-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAG-3 antibody (16616-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

LAG-3/CD223 Polyclonal Antibody for IHC, IF-P, IP, ELISA

Cat No. 16616-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, IP, ELISA

Synonyms

CD223, LAG-3, FDC, LAG 3, LAG3

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Product Information

16616-1-PBS targets LAG-3/CD223 in IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LAG-3/CD223 fusion protein Ag9909
• Full Name: lymphocyte-activation gene 3
• Calculated Molecular Weight: 525 aa, 57 kDa
• Observed Molecular Weight: 54 kDa, 60-65 kDa
• GenBank Accession Number: BC052589
• Gene Symbol: LAG-3
• Gene ID (NCBI): 3902
• RRID: AB_2133350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P18627
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)