WBS15/LAT2 Polyclonal antibody

• 

  WB analysis using 11008-1-AP

  Raji lysates were subjected to SDS PAGE followed by western blot with 11008-1-AP (WBS15/LAT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11008-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11008-1-AP (WBS15/LAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11008-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11008-1-AP (WBS15/LAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

WBS15/LAT2 Polyclonal Antibody for WB, ELISA

Cat No. 11008-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

HSPC046, LAB, LAT2, Non T cell activation linker, NTAL, WBS15, WBSCR15, WBSCR5, WSCR5

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Tested Applications

• Positive WB detected in: Raji cells, human placenta tissue, human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11008-1-AP targets WBS15/LAT2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1454

  Product name: Recombinant human LAT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-243 aa of BC009204

  Sequence: MSSGTELLWPGAALLVLLGVAASLCVRCSRPGAKRSEKIYQQRSLREDQQSFTGSRTYSLVGQAWPGPLADMAPTRKDKLLQFYPSLEDPASSRYQNFSKGSRHGSEEAYIDPIAMEYYNWGRFSKPPEDDDANSYENVLICKQKTTETGAQQEGIGGLCRGDLSLSLALKTGPTSGLCPSASPEEDEESEDYQNSASIHQWRESRKVMGQLQREASPGPVGSPDEEDGEPDYVNGEVAATEA

• Full Name: linker for activation of T cells family, member 2
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC009204
• Gene Symbol: LAT2
• Gene ID (NCBI): 7462
• RRID: AB_2133252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9GZY6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WBS15, also named as LAB, NTAL, WBSCR15, WBSCR5and LAT2 (Linker for activation of T-cells family member 2; not SLC7A8/LAT2), is involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast and myeloid cells. WBS15 is involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells. It is located in the Williams-Beuren syndrome (WBS) critical region. WBS15 has two isoform with MW 27-30 kDa and 12 kDa.