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LATS1 Polyclonal antibody

LATS1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17049-1-AP

Synonyms

h warts, LATS1, WARTS, WARTS protein kinase, wts



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, SH-SY5Y cells, HT-29 cells, A549 cells, C2C12 cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17049-1-AP targets LATS1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LATS1 fusion protein Ag10709
Full Name LATS, large tumor suppressor, homolog 1 (Drosophila)
Calculated molecular weight 15 kDa, 127 kDa
Observed molecular weight 140-150 kDa, 120 kDa
GenBank accession numberBC015665
Gene symbol LATS1
Gene ID (NCBI) 9113
RRIDAB_2281011
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongs to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).

Protocols

Product Specific Protocols
WB protocol for LATS1 antibody 17049-1-APDownload protocol
IHC protocol for LATS1 antibody 17049-1-APDownload protocol
IF protocol for LATS1 antibody 17049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Res

A phosphatidic acid-binding lncRNA SNHG9 facilitates LATS1 liquid-liquid phase separation to promote oncogenic YAP signaling.

Authors - Rui-Hua Li
humanWB

Nat Commun

Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.

Authors - Shujie Chen
humanWB

Theranostics

CSRP2 suppresses colorectal cancer progression via p130Cas/Rac1 axis-meditated ERK, PAK, and HIPPO signaling pathways.

Authors - Lixia Chen
IHC

JCI Insight

KIBRA upregulation increases susceptibility to glomerular injury and correlates with kidney function decline

Authors - Kristin Meliambro
WB

Oncogene

Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.

Authors - Yongyun Li
IHC,WB

Acta Pharmacol Sin

KLF4 initiates sustained YAP activation to promote renal fibrosis in mice after ischemia-reperfusion kidney injury.

Authors - Dan Xu