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LATS1 Polyclonal antibody

LATS1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17049-1-AP

Synonyms

h warts, LATS1, WARTS, WARTS protein kinase, wts



Tested Applications

Positive WB detected inC2C12 cells, mouse testis tissue, K-562 cells, mouse cerebellum tissue, SH-SY5Y cells, HepG2 cells, HT-29 cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17049-1-AP targets LATS1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LATS1 fusion protein Ag10709
Full Name LATS, large tumor suppressor, homolog 1 (Drosophila)
Calculated molecular weight 15 kDa, 127 kDa
Observed molecular weight 140-150 kDa, 120 kDa
GenBank accession numberBC015665
Gene symbol LATS1
Gene ID (NCBI) 9113
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongs to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).

Protocols

Product Specific Protocols
WB protocol for LATS1 antibody 17049-1-APDownload protocol
IHC protocol for LATS1 antibody 17049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
sheepWB,IHC,IF

Theriogenology

Expression of Hippo signaling pathway components in Hu sheep male reproductive tract and spermatozoa.

Authors - Guo-Min Zhang
humanWB

Cells

Myricetin Suppresses the Propagation of Hepatocellular Carcinoma via Down-Regulating Expression of YAP.

Authors - Minjing Li
humanIHC

Cell Death Dis

Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway.

Authors - Yang Han
humanWB

Oncotarget

Transforming growth factor-beta1 suppresses hepatocellular carcinoma proliferation via activation of Hippo signaling.

Authors - Xiaodong Zhang
humanWB

Front Oncol

FAM83A Promotes Lung Cancer Progression by Regulating the Wnt and Hippo Signaling Pathways and Indicates Poor Prognosis.

Authors - Yi-Wen Zheng
mouseIHC,WB

Acta Pharmacol Sin

KLF4 initiates sustained YAP activation to promote renal fibrosis in mice after ischemia-reperfusion kidney injury.

Authors - Dan Xu