LATS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LATS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, RIP, ELISA

Conjugate

Unconjugated

Publications

51

Cat no : 17049-1-AP

Synonyms

h warts, LATS1, WARTS, WARTS protein kinase, wts

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Validation Data Gallery

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  WB analysis of HepG2 using 17049-1-AP

  WB result of LATS1 antibody (17049-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LATS1 transfected HepG2 cells.

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  WB analysis using 17049-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17049-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17049-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 17049-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17049-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17049-1-AP (LATS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17049-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17049-1-AP (LATS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 17049-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17049-1-AP (LATS1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17049-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17049-1-AP (LATS1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 17049-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LATS1 antibody (17049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 17049-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LATS1 antibody (17049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, SH-SY5Y cells, HT-29 cells, A549 cells, C2C12 cells
• Positive IHC detected in: human breast cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 44 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• RIP: See 1 publications below

Product Information

17049-1-AP targets LATS1 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LATS1 fusion protein Ag10709
• Full Name: LATS, large tumor suppressor, homolog 1 (Drosophila)
• Calculated Molecular Weight: 15 kDa, 127 kDa
• Observed Molecular Weight: 140-150 kDa, 120 kDa
• GenBank Accession Number: BC015665
• Gene Symbol: LATS1
• Gene ID (NCBI): 9113
• RRID: AB_2281011
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongs to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).

Publications

Species	Application	Title
human	IF	Cell Res; A phosphatidic acid-binding lncRNA SNHG9 facilitates LATS1 liquid-liquid phase separation to promote oncogenic YAP signaling.; Authors - Rui-Hua Li
human	WB	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen
human	WB	EMBO J; MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression; Authors - Yan Sun
human	WB	Theranostics; CSRP2 suppresses colorectal cancer progression via p130Cas/Rac1 axis-meditated ERK, PAK, and HIPPO signaling pathways.; Authors - Lixia Chen
	IHC	JCI Insight; KIBRA upregulation increases susceptibility to glomerular injury and correlates with kidney function decline; Authors - Kristin Meliambro
	WB	Oncogene; Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.; Authors - Yongyun Li