Validation Data Gallery
|Positive WB detected in||HepG2 cells, K-562 cells, SH-SY5Y cells, HT-29 cells, A549 cells, C2C12 cells|
|Positive IHC detected in||human breast cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17049-1-AP targets LATS1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LATS1 fusion protein Ag10709|
|Full Name||LATS, large tumor suppressor, homolog 1 (Drosophila)|
|Calculated molecular weight||15 kDa, 127 kDa|
|Observed molecular weight||140-150 kDa, 120 kDa|
|GenBank accession number||BC015665|
|Gene ID (NCBI)||9113|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongs to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).
Expression of Hippo signaling pathway components in Hu sheep male reproductive tract and spermatozoa.
Myricetin Suppresses the Propagation of Hepatocellular Carcinoma via Down-Regulating Expression of YAP.
Cell Death Dis
Alginate oligosaccharide attenuates α2,6-sialylation modification to inhibit prostate cancer cell growth via the Hippo/YAP pathway.
Transforming growth factor-beta1 suppresses hepatocellular carcinoma proliferation via activation of Hippo signaling.
FAM83A Promotes Lung Cancer Progression by Regulating the Wnt and Hippo Signaling Pathways and Indicates Poor Prognosis.
Acta Pharmacol Sin
KLF4 initiates sustained YAP activation to promote renal fibrosis in mice after ischemia-reperfusion kidney injury.