LCTL Polyclonal antibody

LCTL Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24589-1-AP

Synonyms

KLG, KLPH, lactase like, Lactase like protein, LCTL

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Validation Data Gallery

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  WB analysis of MCF-7 using 24589-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24589-1-AP (LCTL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 24589-1-AP

  Immunofluorescent analysis of HeLa cells using 24589-1-AP (LCTL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24589-1-AP targets LCTL in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LCTL fusion protein Ag20220
• Full Name: lactase-like
• Calculated Molecular Weight: 567 aa, 65 kDa
• Observed Molecular Weight: 39 kDa, 65 kDa, 70 kDa
• GenBank Accession Number: BC152990
• Gene Symbol: LCTL
• Gene ID (NCBI): 197021
• RRID: AB_2879625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lactase-like is a glycosidase enzyme, which could hydrolyze glycosidic bonds and are classified into families based on primary amino acid sequence. The mouse ortholog of this protein has been characterized and has a domain structure of an N-terminal signal peptide, glycosidase domain, transmembrane domain, and a short cytoplasmic tail.