LIMS1 Polyclonal antibody, PBS Only

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  WB analysis of HeLa using 20772-1-AP (same clone as 20772-1-PBS)

  WB result of LIMS1 antibody (20772-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMS1 transfected HeLa cells. This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

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  WB analysis using 20772-1-AP (same clone as 20772-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

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  WB analysis of Caco-2 using 20772-1-AP (same clone as 20772-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 20772-1-AP (same clone as 20772-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 20772-1-AP (same clone as 20772-1-PBS)

  IP result of anti-LIMS1 (IP:20772-1-AP, 4ug; Detection:20772-1-AP 1:800) with K-562 cells lysate 1640 ug. This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 20772-1-AP (same clone as 20772-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20772-1-AP (LIMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 20772-1-AP (same clone as 20772-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20772-1-AP (LIMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20772-1-AP (same clone as 20772-1-PBS)

  Immunofluorescent analysis of Hela cells, using LIMS1 antibody 20772-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20772-1-AP (same clone as 20772-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LIMS1 antibody (20772-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

LIMS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 20772-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, PINCH, PINCH 1, PINCH1

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Product Information

20772-1-PBS targets LIMS1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14764

  Product name: Recombinant human LIMS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-325 aa of BC005341

  Sequence: MANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFTIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGGVVSALNKAWCVNCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLAETLGRK

• Full Name: LIM and senescent cell antigen-like domains 1
• Calculated Molecular Weight: 325 aa, 37 kDa
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: BC005341
• Gene Symbol: LIMS1
• Gene ID (NCBI): 3987
• RRID: AB_10695765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48059
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LIMS1, also known as PINCH1, is an adaptor protein that contains five LIM domains, or double zinc fingers(PMID: 7517666). PINCH-1 is a widely expressed focal adhesion protein that forms a ternary complex with integrin-linked kinase (ILK) and CH-ILKBP/actopaxin/alpha-parvin (PMID: 14551191). LIMS1 is involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques.