Tested Applications
| Positive WB detected in | Caco-2 cells, human kidney tissue, HeLa cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20772-1-AP targets LIMS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14764 Product name: Recombinant human LIMS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC005341 Sequence: MANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFTIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGGVVSALNKAWCVNCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLAETLGRK Predict reactive species |
| Full Name | LIM and senescent cell antigen-like domains 1 |
| Calculated Molecular Weight | 325 aa, 37 kDa |
| Observed Molecular Weight | 35-37 kDa |
| GenBank Accession Number | BC005341 |
| Gene Symbol | LIMS1 |
| Gene ID (NCBI) | 3987 |
| RRID | AB_10695765 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48059 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LIMS1, also known as PINCH1, is an adaptor protein that contains five LIM domains, or double zinc fingers(PMID: 7517666). PINCH-1 is a widely expressed focal adhesion protein that forms a ternary complex with integrin-linked kinase (ILK) and CH-ILKBP/actopaxin/alpha-parvin (PMID: 14551191). LIMS1 is involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LIMS1 antibody 20772-1-AP | Download protocol |
| IHC protocol for LIMS1 antibody 20772-1-AP | Download protocol |
| IP protocol for LIMS1 antibody 20772-1-AP | Download protocol |
| WB protocol for LIMS1 antibody 20772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PINCH-1 antibody (Cat# 20772-1-AP) has 8 citations spanning journals including Nature Communications, Theranostics, Biomaterials, Journal of Cell Biology, Journal of Thrombosis and Haemostasis, Journal of Cell Science, Cancer Medicine, and Amino Acids. Research fields covered include tumor growth and metastasis, mitochondrial dynamics, skin and pancreatic cancer progression, stem cell differentiation, arterial thrombosis, integrin activation, colon cancer, and metabolic reprogramming. Most studies applied the antibody for Western blot and immunoprecipitation in human samples.
| Species | Application | Title |
|---|---|---|
Nat Commun PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.
| ||
Theranostics PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex. | ||
Biomaterials PINCH-1-dependent regulation on tumor matrix microenvironment in pancreatic cancer.
| ||
J Cell Biol A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.
| ||
J Thromb Haemost Red blood cell-derived semaphorin 7A accelerates carotid arterial thrombosis under disturbed flow via interacting with platelet α(IIb)β(3). | ||
J Cell Sci Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation. |
Reviews
What customers say
The single review for this product is highly positive, giving it a 5-star rating. The reviewer used it for Western Blot on HeLa and NIH3T3 cells at a 1:1000 dilution and described it as an excellent antibody that works well at that dilution. Overall, the feedback highlights strong performance and reliability for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Yasmin (Verified Customer) (08-01-2019) | Excellent antibody for western blot. Works well at 1:1000 dilution.
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