LRCH2 Polyclonal antibody

LRCH2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 24866-1-AP

Synonyms

dA204F4.4, KIAA1495, LRCH2

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Validation Data Gallery

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  WB analysis of mouse brain using 24866-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24866-1-AP (LRCH2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 24866-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 24866-1-AP (LRCH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 24866-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 24866-1-AP (LRCH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 24866-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24866-1-AP (LRCH2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 24866-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24866-1-AP (LRCH2 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue, mouse ovary tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24866-1-AP is LRCH2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRCH2 fusion protein Ag21701
• Full Name: leucine-rich repeats and calponin homology (CH) domain containing 2
• Calculated molecular weight: 765 aa, 85 kDa
• Observed molecular weight: 84 kDa
• GenBank accession number: BC125224
• Gene symbol: LRCH2
• Gene ID (NCBI): 57631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Leucine rich repeat and calponin homology domain containing protein 2 (LRCH2) also named as KIAA1495 is a 765 amino acid protein, which contains one CH domain and nine LRR repeats. The function of LRCH2 is still non-known.