LRP5 Polyclonal antibody

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  WB analysis of HeLa using 24899-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24899-1-AP (LRP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 24899-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24899-1-AP (LRP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 24899-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24899-1-AP (LRP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 24899-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 24899-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 24899-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 24899-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 24899-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24899-1-AP (LRP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

LRP5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24899-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

BMND1, EVR1, EVR4, HBM, LR3, LRP 5, LRP5, LRP7, OPPG, OPS, OPTA1, VBCH2

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: mouse lung tissue, human hepatocirrhosis tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

24899-1-AP targets LRP5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRP5 fusion protein Ag19034
• Full Name: low density lipoprotein receptor-related protein 5
• Calculated Molecular Weight: 1615 aa, 179 kDa
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: BC150595
• Gene Symbol: LRP5
• Gene ID (NCBI): 4041
• RRID: AB_2879786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75197
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Low-density lipoprotein receptor-related protein 5 (LRP5) is a single-pass type I membrane protein with EGF-like and LDLR domains in the extracellular domain. LRP5 and LRP6 act as coreceptors with Frizzled for Wnt. The complex of Wnt-Fzd-LRP5-LRP6 triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. LRP5 plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in LRP5 gene.

Publications

Species	Application	Title
human	WB	Stem Cell Res Ther; LINC01119 negatively regulates osteogenic differentiation of mesenchymal stem cells via the Wnt pathway by targeting FZD4.; Authors - Hongwei Gao
mouse	WB,IHC,IF,IP	Front Pharmacol; Sini San Inhibits Chronic Psychological Stress-Induced Breast Cancer Stemness by Suppressing Cortisol-Mediated GRP78 Activation.; Authors - Yifeng Zheng
mouse	WB	Calcif Tissue Int; Coenzyme Q10 Protects Against Hyperlipidemia-Induced Osteoporosis by Improving Mitochondrial Function via Modulating miR-130b-3p/PGC-1α Pathway; Authors - Meng Meng
rat	WB	Pharm Biol; Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl4/2-AAF.; Authors - Ying Xu
human	IHC, WB	Chin J Integr Med; Babao Dan () Alleviates 5-Fluorouracil-Induced Intestinal Damage via Wnt/β-Catenin Pathway.; Authors - Meng-Xuan Gui
human	IHC	J Orthop Sci; Wnt signaling pathway correlates with ossification of the spinal ligament: A microRNA array and immunohistochemical study.; Authors - Takafumi Yayama