LRP5 Polyclonal antibody

LRP5 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 24899-1-AP

Synonyms

BMND1, EVR1, EVR4, HBM, LR3, LRP 5, LRP5, LRP7, OPPG, OPS, OPTA1, VBCH2



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human hepatocirrhosis tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24899-1-AP is LRP5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRP5 fusion protein Ag19034
Full Name low density lipoprotein receptor-related protein 5
Calculated molecular weight 1615 aa, 179 kDa
Observed molecular weight 180-200 kDa
GenBank accession numberBC150595
Gene symbol LRP5
Gene ID (NCBI) 4041
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Low-density lipoprotein receptor-related protein 5 (LRP5) is a single-pass type I membrane protein with EGF-like and LDLR domains in the extracellular domain. LRP5 and LRP6 act as coreceptors with Frizzled for Wnt. The complex of Wnt-Fzd-LRP5-LRP6 triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. LRP5 plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in LRP5 gene.

Protocols

Product Specific Protocols
WB protocol for LRP5 antibody 24899-1-APDownload protocol
IHC protocol for LRP5 antibody 24899-1-APDownload protocol
IF protocol for LRP5 antibody 24899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Orthop Sci

Wnt signaling pathway correlates with ossification of the spinal ligament: A microRNA array and immunohistochemical study.

Authors - Takafumi Yayama
humanIHC, WB

Chin J Integr Med

Babao Dan () Alleviates 5-Fluorouracil-Induced Intestinal Damage via Wnt/β-Catenin Pathway.

Authors - Meng-Xuan Gui