LRPPRC Recombinant monoclonal antibody, PBS Only (Detector)

• 

  WB analysis using 86616-2-RR (same clone as 86616-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86616-2-RR (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86616-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP02559-1

  The mean LRPPRC concentration was determined to be 67.2 ng/mL in HeLa cell extract based on a 1.1 mg/mL extract load, 168.4 ng/mL in HCT 116 cell extract based on a 1.3 mg/mL extract load.

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  Cytometric bead array standard curve of MP02559-1

  Cytometric bead array standard curve of MP02559-1, LRPPRC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86616-1-PBS. Detection antibody: 86616-2-PBS. Standard: Ag15452. Range: 1.562-100 ng/mL.

LRPPRC Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 86616-2-PBS

Clone No.251577F1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

130 kDa leucine-rich protein, GP130, Leucine-rich PPR motif-containing protein, mitochondrial, LRP 130, LRP130

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Product Information

86616-2-PBS targets LRPPRC as part of a matched antibody pair:

MP02559-1: 86616-1-PBS capture and 86616-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15452

  Product name: Recombinant human LRPPRC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1014-1394 aa of BC050311

  Sequence: LWYEDEKHSLNSSSASTTEPDFQKDILIACRLNQKKGAYDIFLNAKEQNIVFNAETYSNLIKLLMSEDYFTQAMEVKAFAETHIKGFTLNDAANSRLIITQVRRDYLKEAVTTLKTVLDQQQTPSRLAVTRVIQALAMKGDVENIEVVQKMLNGLEDSIGLSKMVFINNIALAQIKNNNIDAAIENIENMLTSENKVIEPQYFGLAYLFRKVIEEQLEPAVEKISIMAERLANQFAIYKPVTDFFLQLVDAGKVDDARALLQRCGAIAEQTPILLLFLLRNSRKQGKASTVKSVLELIPELNEKEEAYNSLMKSYVSEKDVTSAKALYEHLTAKNTKLDDLFLKRYASLLKYAGEPVPFIEPPESFEFYAQQLRKLRENSS

• Full Name: leucine-rich PPR-motif containing
• Calculated Molecular Weight: 1394 aa, 158 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC050311
• Gene Symbol: LRPPRC
• Gene ID (NCBI): 10128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42704
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria, where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.