LRRC56 Polyclonal antibody

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  IHC staining of mouse brain using 27796-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27796-1-AP (LRRC56 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 27796-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27796-1-AP (LRRC56 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 27796-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LRRC56 antibody (27796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

LRRC56 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 27796-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

leucine rich repeat containing 56

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Tested Applications

• Positive IHC detected in: mouse brain tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27796-1-AP targets LRRC56 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRRC56 fusion protein Ag22018
• Full Name: leucine rich repeat containing 56
• Calculated Molecular Weight: 542 aa, 59 kDa
• GenBank Accession Number: BC035936
• Gene Symbol: LRRC56
• Gene ID (NCBI): 115399
• RRID: AB_3669624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IYG6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Leucine-rich repeat (LRR)-containing gene 56 (LRRC56) was recently reported to be a homolog of the Outer Dynein Arm 8 gene (oda8), a gene necessary for the cytoplasmic maturation of the outer dynein arms (ODA) in the flagella of algae. LRRC56 is recruited during axoneme construction in an IFT-dependent manner and is required for the addition of dynein arms to the distal segment of the flagellum. Human LRRC56 encodes a 542 amino acid protein determined from transcriptomic studies and immunohistochemistry to be expressed in many organs, mostly in testis and pituitary gland.