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  • KD/KO Validated

LY6E Polyclonal antibody

LY6E Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22144-1-AP

Synonyms

9804, Ly 6E, LY6E, Lymphocyte antigen 6E, RIG E, RIGE, SCA 2, SCA2, Stem cell antigen 2, Thymic shared antigen 1, TSA 1, TSA1



Tested Applications

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

22144-1-AP targets LY6E in WB, IHC, IF, ELISA applications and shows reactivity with human, Mouse samples.

Tested Reactivity human, Mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LY6E fusion protein Ag17573
Full Name lymphocyte antigen 6 complex, locus E
Calculated molecular weight 131 aa, 14 kDa
Observed molecular weight12-16 kDa
GenBank accession numberBC119708
Gene symbol LY6E
Gene ID (NCBI) 4061
RRIDAB_2879007
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The predicted MW of LY6E is 13 kDa. Catalog#22144-1-AP recognises two band of 13 kDa and 18 kDa, and the additional 18 kDa band may be due to glycosylation.

Protocols

Product Specific Protocols
WB protocol for LY6E antibody 22144-1-APDownload protocol
IHC protocol for LY6E antibody 22144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Lab Chip

LY6E protein facilitates adeno-associated virus crossing in a biomimetic chip model of the human blood-brain barrier

Authors - Dan Liu
humanWB,IHC

Carcinogenesis

α5-nAChR associated with Ly6E modulates cell migration via TGF-β1/Smad signaling in non-small cell lung cancer.

Authors - Qian Zhang
humanWB

J Virol

LY6E Restricts the Entry of Human Coronaviruses, Including the Currently Pandemic SARS-CoV-2.

Authors - Xuesen Zhao
  • KD Validated
humanWB

J Virol

Characterization of CCoV-HuPn-2018 spike protein-mediated viral entry

Authors - Yongmei Liu