|Positive WB detected in
|mouse kidney tissue
|Positive IHC detected in
|human tonsillitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:200-1:1000
|IHC : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
22144-1-AP targets LY6E in WB, IHC, IF, ELISA applications and shows reactivity with human, Mouse samples.
|Host / Isotype
|Rabbit / IgG
|LY6E fusion protein Ag17573
|lymphocyte antigen 6 complex, locus E
|Calculated molecular weight
|131 aa, 14 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The predicted MW of LY6E is 13 kDa. Catalog#22144-1-AP recognises two band of 13 kDa and 18 kDa, and the additional 18 kDa band may be due to glycosylation.
LY6E protein facilitates adeno-associated virus crossing in a biomimetic chip model of the human blood-brain barrier
α5-nAChR associated with Ly6E modulates cell migration via TGF-β1/Smad signaling in non-small cell lung cancer.
LY6E Restricts the Entry of Human Coronaviruses, Including the Currently Pandemic SARS-CoV-2.
Characterization of CCoV-HuPn-2018 spike protein-mediated viral entry