Cat no : lcg
DNA plasmid encoding for anti-Lamin VHH (anti-Lamin Nanobody) fused to TagGFP2.
|Applications||Live cell imaging|
|Specificity||Lamin A/C, tested in human cell lines (HeLa, U2OS) and insect S2 cells.|
|Vector type||Mammalian expression vector|
|Encoded Nanobody/ VHH||Monoclonal anti-Lamin single domain antibody (sdAb) fragment|
|Sequence||With the Lamin-Chromobody plasmid you receive the sequence information of the Alpaca antibody to Lamin fused to TagGFP2, as well as the full vector sequence.|
|Microscopy techniques||Wide-field epifluorescence microscopy; confocal microscopy|
|Transfection||Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.|
|Storage Condition||Shipped at ambient temperature. Store at -20 °C|
A Strategy to Optimize the Generation of Stable Chromobody Cell Lines for Visualization and Quantification of Endogenous Proteins in Living Cells.
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GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics.
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A transient pool of nuclear F-actin at mitotic exit controls chromatin organization.