Livin Polyclonal antibody, PBS Only

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  WB analysis using 27543-1-AP (same clone as 27543-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27543-1-AP (Livin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 27543-1-AP (same clone as 27543-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 27543-1-AP (Livin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 27543-1-AP (same clone as 27543-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 27543-1-AP (Livin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27543-1-AP (same clone as 27543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27543-1-AP (Livin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27543-1-AP (same clone as 27543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27543-1-AP (Livin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27543-1-PBS in a different storage buffer formulation.

Livin Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 27543-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

27543-1-PBS targets Livin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Livin fusion protein Ag26737
• Full Name: baculoviral IAP repeat-containing 7
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC014475
• Gene Symbol: Livin
• Gene ID (NCBI): 79444
• RRID: AB_2880901
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96CA5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BIRC7 also known as Livin, is one of the family members of the inhibitor of apoptosis protein (IAP). BIRC7 contains a BIR domain that has been shown to bind and inhibit caspases-3, −7 and −9, playing a key role in inhibiting apoptosis. Overexpression of BIRC7 in tumors has been linked with increased chemo- and radio-resistance, recurrence, and decreased patient survival. BIRC7 is closely associated with melanoma disease progression, apoptosis resistance, and chemotherapeutic drug resistance.