Livin Polyclonal antibody

Livin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 27543-1-AP

Synonyms

BIRC7, KIAP, LIVIN, ML IAP, MLIAP, RING finger protein 50, RNF50

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Validation Data Gallery

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  WB analysis using 27543-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27543-1-AP (Livin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 27543-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27543-1-AP (Livin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 27543-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 27543-1-AP (Livin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27543-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27543-1-AP (Livin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27543-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27543-1-AP (Livin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, A431 cells, A375 cells, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

27543-1-AP targets Livin in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Livin fusion protein Ag26737
• Full Name: baculoviral IAP repeat-containing 7
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC014475
• Gene Symbol: BIRC7
• Gene ID (NCBI): 79444
• RRID: AB_2880901
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BIRC7 also known as Livin, is one of the family members of the inhibitor of apoptosis protein (IAP). BIRC7 contains a BIR domain that has been shown to bind and inhibit caspases-3, −7 and −9, playing a key role in inhibiting apoptosis. Overexpression of BIRC7 in tumors has been linked with increased chemo- and radio-resistance, recurrence, and decreased patient survival. BIRC7 is closely associated with melanoma disease progression, apoptosis resistance, and chemotherapeutic drug resistance.

Publications

Species	Application	Title
human	WB	Int J Oncol; Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.; Authors - Wei Jia
human	IHC	BMC Cancer; The clinicopathological and prognostic significances of IGF-1R and Livin expression in patients with colorectal cancer.; Authors - Zhenling Zhang
human	WB	Exp Physiol; Oncostatin M upregulates Livin to promote keratinocyte proliferation and survival via ERK and STAT3 signalling pathways.; Authors - Hao Wang
human	WB	Bioengineered; Down-regulation of lncRNA DNAJC3-AS1 inhibits colon cancer via regulating miR-214-3p/LIVIN axis.; Authors - Bing Han