MAEA Polyclonal antibody

MAEA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28363-1-AP

Synonyms

EMLP, EMP, HLC 10, MAEA, PIG5



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28363-1-AP targets MAEA in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAEA fusion protein Ag27150
Full Name macrophage erythroblast attacher
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC001225
Gene Symbol MAEA
Gene ID (NCBI) 10296
RRIDAB_2881122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).

Protocols

Product Specific Protocols
WB protocol for MAEA antibody 28363-1-APDownload protocol
IHC protocol for MAEA antibody 28363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway.

Authors - Chunyuan Tang
humanWB

Oncogene

E3 ligase MAEA-mediated ubiquitination and degradation of PHD3 promotes glioblastoma progression

Authors - Peijun Zhou
  • KD Validated
humanWB

Chembiochem

Identifying E3 ligase substrates with quantitative degradation proteomics

Authors - Victoria N Jordan
  • KO Validated
humanWB,IHC

J Transl Med

LAD1 promotes malignant progression by diminishing ubiquitin-dependent degradation of vimentin in gastric cancer

Authors - Yingming Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

S (Verified Customer) (12-31-2021)

Quite a good quality antibody. KO validated.

  • Applications: Western Blot
  • Cell Tissue Type: HEK293T
MAEA Antibody Western Blot validation ( dilution) in HEK293T (Cat no:28363-1-AP)
FH

H (Verified Customer) (07-09-2020)

This is very good antibody. MAEA band is very thick and clear.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
MAEA Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:28363-1-AP)