MAGED1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MAGED1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 22053-1-AP

Synonyms

PP2250, NRAGE, Neurotrophin receptor-interacting MAGE homolog, Melanoma-associated antigen D1, MAGE-D1 antigen

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Validation Data Gallery

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  WB analysis of A549 using 22053-1-AP

  WB result of MAGED1 antibody (22053-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAGED1 transfected A549 cells.

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  WB analysis of A549 using 22053-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22053-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22053-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 22053-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 22053-1-AP

  IP result of anti-MAGED1 (IP:22053-1-AP, 4ug; Detection:22053-1-AP 1:1000) with A549 cells lysate 400ug.

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  IHC staining of mouse placenta using 22053-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 22053-1-AP (MAGED1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 22053-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 22053-1-AP (MAGED1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 22053-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 22053-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 22053-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 22053-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 22053-1-AP

  Immunofluorescent analysis of A549 cells using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 22053-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAGED1 Polyclonal antibody (22053-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, mouse placenta tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: mouse placenta tissue, human skin tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

22053-1-AP targets MAGED1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGED1 fusion protein Ag17047
• Full Name: melanoma antigen family D, 1
• Calculated Molecular Weight: 834 aa, 92 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC014070
• Gene Symbol: MAGED1
• Gene ID (NCBI): 9500
• RRID: AB_11182601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGED1 (also known as NRAGE or Dlxin-1) is a member of the type II melanoma antigen (MAGE) family of protein. MAGED1 can interact with the nerve growth factor receptor p75NTR, antagonize the association of p75NTR with TrkA, inhibit cell cycle progression, and facilitate p75NTR-mediated apoptosis (PMID: 10985348). It has been reported that MAGED1 binds Dlx5 and regulates its transcriptional function. In vivo, MAGED1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homomultimers (PMID: 11084035). MAGED1 knockout mice show depressive behavior and impairments of circadian rhythm (PMID: 20300063; 23314527). Three transcript variants encoding two different isoforms (86kDa and 92 kDa) have been found for the gene of MAGED1.

Publications

Species	Application	Title
mouse	WB	Clin Transl Med; Saikosaponin-d impedes hippocampal neurogenesis and causes cognitive deficits by inhibiting the survival of neural stem/progenitor cells via neurotrophin receptor signaling in mice.; Authors - Tingting Qin
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system.; Authors - Qiang Lu
human	WB,IHC,IF	Tumour Biol; Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells.; Authors - Huandi Zhou ; KD Validated
human	WB,IHC	Gene; NRAGE confers poor prognosis and promotes proliferation, invasion, and chemoresistance in gastric cancer.; Authors - Xiaodi Jiang ; KD Validated
human	WB	bioRxiv; Histone chaperone HIRA, Promyelocytic Leukemia (PML) protein and p62/SQSTM1 coordinate to regulate inflammation during cell senescence; Authors - Nirmalya Dasgupta